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http://dx.doi.org/10.5483/BMBRep.2019.52.5.155

MiR-371 promotes proliferation and metastasis in hepatocellular carcinoma by targeting PTEN  

Wang, Hao (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University)
Zhao, Yi (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University)
Chen, Tingsong (The Seventh People's Hospital of Shanghai)
Liu, Guofang (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University)
He, Nan (Guangdong Ascendas Genomics Technology Co., Ltd.)
Hu, Heping (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University)
Publication Information
BMB Reports / v.52, no.5, 2019 , pp. 312-317 More about this Journal
Abstract
Hepatocellular carcinoma (HCC) is the leading cause of cancer-related mortality worldwide. MiR-371 has recently emerged as an important regulator in tumorigenesis, and may serve as a biomarker for malignant tumors. We transfected miR-371 or its inhibitor in two human HCC cell lines, then used 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, soft agar colony formation, and transwell migration assays to evaluate the effects on cell proliferation, migration, and invasion. We found that miR-371 was positively correlated with HCC metastasis and poor prognosis in the inflicted patients, and the high expression of miR-371 was promoted, whereas a low level of miR-371 depressed cell proliferation and invasion. We found PTEN to be a direct target of miR-371. The overexpression or knockdown of PTEN exhibited the opposite effects from those of miR-371 on cell proliferation and migration. Our study demonstrates that miR-371 promotes proliferation and metastasis in HCC by targeting PTEN.
Keywords
Cancer; Hepatocellular carcinoma; Metastasis; miR-371; PTEN;
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