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http://dx.doi.org/10.5483/BMBRep.2019.52.10.182

Deficiency of calpain-6 inhibits primary ciliogenesis  

Kim, Bo Hye (Department of Biological Sciences, Sookmyung Women's University)
Kim, Do Yeon (Department of Biological Sciences, Sookmyung Women's University)
Oh, Sumin (Department of Biological Sciences, Sookmyung Women's University)
Ko, Je Yeong (Department of Biological Sciences, Sookmyung Women's University)
Rah, Gyuyeong (Department of Biological Sciences, Sookmyung Women's University)
Yoo, Kyung Hyun (Department of Biological Sciences, Sookmyung Women's University)
Park, Jong Hoon (Department of Biological Sciences, Sookmyung Women's University)
Publication Information
BMB Reports / v.52, no.10, 2019 , pp. 619-624 More about this Journal
Abstract
The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding human orthologs, with significantly upregulated expression at 24 h after serum withdrawal. Finally, calpain-6 was selected as a positive regulator of ciliogenesis. We found that calpain-6 deficiency reduced the percentage of ciliated cells and impaired sonic hedgehog signaling. It has been speculated that this defect might be associated with decreased levels of ${\alpha}-tubulin$ acetylation at lysine 40. This is the first study to report a novel role of calpain-6 in the formation of primary cilia.
Keywords
Acetylated ${\alpha}-tubulin$; Calpain-6; mRNA microarray; Primary cilia; Tubulin code;
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