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http://dx.doi.org/10.5483/BMBRep.2018.51.9.153

Potential involvement of Drosophila flightless-1 in carbohydrate metabolism  

Park, Jung-Eun (Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center)
Jang, Jinho (Department of Biological Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology)
Lee, Eun Ji (Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center)
Kim, Su Jung (Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine)
Yoo, Hyun Ju (Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine)
Lee, Semin (Department of Biological Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology)
Kang, Min-Ji (Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center)
Publication Information
BMB Reports / v.51, no.9, 2018 , pp. 462-467 More about this Journal
Abstract
A previous study of ours indicated that Drosophila flightless-1 controls lipid metabolism, and that there is an accumulation of triglycerides in flightless-1 (fliI)-mutant flies, where this mutation triggers metabolic stress and an obesity phenotype. Here, with the aim of characterizing the function of FliI in metabolism, we analyzed the levels of gene expression and metabolites in fliI-mutant flies. The levels of enzymes related to glycolysis, lipogenesis, and the pentose phosphate pathway increased in fliI mutants; this result is consistent with the levels of metabolites corresponding to a metabolic pathway. Moreover, high-throughput RNA sequencing revealed that Drosophila FliI regulates the expression of genes related to biological processes such as chromosome organization, carbohydrate metabolism, and immune reactions. These results showed that Drosophila FliI regulates the expression of metabolic genes, and that dysregulation of the transcription controlled by FliI gives rise to metabolic stress and problems in the development and physiology of Drosophila.
Keywords
Flightless-1; Gene expression; Metabolism; Metabolomics; RNA sequencing;
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