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http://dx.doi.org/10.5483/BMBRep.2018.51.7.101

Protective effects of Tat-DJ-1 protein against streptozotocin-induced diabetes in a mice model  

Yeo, Hyeon Ji (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Yeo, Eun Ji (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Shin, Min Jea (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Choi, Yeon Joo (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Lee, Chi Hern (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Kwon, Hyeok Yil (Department of Physiology, College of Medicine, Hallym University)
Kim, Dae Won (Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University)
Eum, Won Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Choi, Soo Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Publication Information
BMB Reports / v.51, no.7, 2018 , pp. 362-367 More about this Journal
Abstract
A major feature of type 1 diabetes mellitus (T1DM) is hyperglycemia and dysfunction of pancreatic ${\beta}$-cells. In a previous study, we have shown that Tat-DJ-1 protein inhibits pancreatic RINm5F ${\beta}$-cell death caused by oxidative stress. In this study, we examined effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic ${\beta}$-cell destruction and regulated levels of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion. In addition, transduced WT Tat-DJ-1 protein significantly inhibited the activation of $NF-{\kappa}B$ and MAPK (ERK and p38) expression as well as expression of COX-2 and iNOS in STZ exposed pancreas. In contrast, treatment with C106A mutant Tat-DJ-1 protein showed no protective effects. Collectively, our results indicate that WT Tat-DJ-1 protein can significantly ameliorate pancreatic tissues in STZ-induced diabetes in mice.
Keywords
Blood glucose; Diabetes mellitus; Insulin; Protein therapy; Tat-DJ-1;
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1 Shi Y, Wan X, Shao N, Ye R, Zhang N and Zhang Y (2016) Protective and anti-angiopathy effects of ginsenoside Re against diabetes mellitus via the activation of p38MAPK, ERK1/2 and JNK signaling. Mol Med Rep 14, 4849-4856   DOI
2 Zhang Y, Mei H, Shan W et al (2016) Lentinan protects pancreatic ${\beta}$ cells from STZ-induced damage. J Cell Mol Med 20, 1803-1812   DOI
3 Sakai N, Wada T, Furuichi K et al (2005) Involvement of extracellular signal-regulated kinase and p38 in human diabetic nephropathy. Am J Kidney Dis 45, 54-65   DOI
4 Weih F, Carrasco D, Durham SK et al (1995) Multiorgan inflammation and hematopoietic abnormalities in mice with a targeted disruption of RelB, a member of the $NF-{\beta}B$/Rel family. Cell 80, 331-340   DOI
5 Mollah ZH, Pai S, Moore C et al (2008) Abnormal NF-kappa B function characterizes human type 1 diabetes dendritic cells and monocytes. J Immunol 180, 3166-3175   DOI
6 Lundh M, Scully SS, Mandrup-Poulsen T and Wagner BK (2013) Small-molecule inhibition of inflammatory beta cell death. Diabetes Obes Metab 15, 176-184   DOI
7 Donath MY and Shoelson SE (2011) Type 2 diabetes as an inflammatory disease. Nat Rev Immunol 11, 98-107   DOI
8 Rabinovitch A and Suarez-Pinzon WL (1998) Cytokines and their roles in pancreatic islet beta-cell destruction and insulin-dependent diabetes mellitus. Biochem Pharmacol 55, 1139-1149   DOI
9 Kellogg AP, Cheng HT and Pop-Busui R (2008) Cyclooxygenase-2 pathway as a potential therapeutic target in diabetic peripheral neuropathy. Curr Drug Targets 9, 68-76   DOI
10 Bujalska-Zadrozny M, de Corde A and Pawlik K (2015) Influence of nitric oxide synthase or cyclooxygenase inhibitors on cannabinosids activity in streptozotocininduced neuropathy. Pharmacol Rep 67, 209-216   DOI
11 An SY, Youn GS, Kim HJ, Choi SY and Park J (2017) Celastrol suppresses expression of adhesion molecules and chemokines by inhibiting JNK-STAT1/$NF-{\kappa}B$ activation in poly(I:C)-stimulated astrocytes. BMB Rep 50, 25-30   DOI
12 Bujalska M, Tatarkiewicz J, de Corde A and Gumulka SW (2008) Effects of cyclooxygenase and nitric oxide synthase inhibitors on streptozotocin-induced hyperalgesia in rats. Pharmacol 81, 151-157   DOI
13 Ahn EH, Kim DW, Shin MJ et al (2016) Tat-ATOX1 inhibits streptozotocin-induced cell death in pancreatic RINm5F cells and attenuates diabetes in a mouse model. Int J Mol Med 38, 217-224   DOI
14 Yang SJ, Kim J, Lee SE, Ahn JY, Choi SY and Cho SW (2017) Anti-inflammatory and anti-oxidative effects of 3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride on ${\beta}$-amyloid-induced microglial activation. BMB Rep 50, 634-639   DOI
15 Rui L (2014) Energy metabolism in the liver. Compr Physiol 4, 177-197
16 Lukic ML, Pejnovic N and Lukic A (2014) New insight into early events in type 1 diabetes: role for islet stem cell exosomes. Diabetes 63, 835-837   DOI
17 Daneman D (2006) Type 1 diabetes. Lancet 367, 847-858   DOI
18 Welters A and Lammert E (2014) Diabetes Mellitus. In: Lammert E, Zeeb M (eds) Metabolism of human diseases: organ physiology and pathophysiology. Springer, Vienna, 163-173
19 Fiorentino TV, Prioletta A, Zuo P and Folli F (2013) Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases. Curr Pharm Des 19, 5695-5703   DOI
20 Esposito K, Marfella R and Giugliano D (2003) Stress hyperglycemia, inflammation, and cardiovascular events. Diabetes Care 26, 1650-1651
21 Hayashi K, Kojima R and Ito M (2006) Strain differences in the diabetogenic activity of streptozotocin in mice. Biol Pharm Bull 29, 1110-1119   DOI
22 Ariga H, Takahashi-Niki K, Kato I, Maita H, Niki T and Iguchi-Ariga SM (2013) Neuroprotective function of DJ-1 in Parkinson's disease. Oxidative Med Cell Longev 2013, 683920
23 Mikio I, Yoichi K, Akiko N, Koji H and Atsuhiko N (2001) Characterization of low dose streptozotocin-induced progressive diabetes in mice. Environ Toxicol Pharmacol 9, 71-78   DOI
24 Nagakubo D, Taira T, Kitaura H et al (1997) DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem Biophys Res Commun 231, 509-513   DOI
25 Wilson MA (2011) The role of cysteine oxidation in DJ-1 function and dysfunction. Antioxid Redox Signal 15, 111-122   DOI
26 Bonifati V, Rizzu P, van Baren MJ et al (2003) Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism. Science 299, 256-259   DOI
27 Jain D, Jain R, Eberhard D et al (2012) Age- and diet-dependent requirement of DJ-1 for glucose homeostasis in mice with implications for human type 2 diabetes. J Mol Cell Biol 4, 221-230   DOI
28 Yu HH, Xu Q, Chen HP et al (2013) Stable overexpression of DJ-1 protects H9c2 cells against oxidative stress under a hypoxia condition. Cell Biochem Funct 31, 643-651   DOI
29 Inden M, Taira T, Kitamura Y et al (2006) PARK7 DJ-1 protects against degeneration of nigral dopaminergic neurons in Parkinson's disease rat model. Neurobiol Dis 24, 144-158   DOI
30 Kim RH, Smith PD, Aleyashin H et al (2005) Hypersensitive of DJ-1-deficient mice to 1-methtyl-4-phenyl- 1,2,3,6-tetrahydropyrindine (MPTP) and oxidative stress. Proc Natl Acad Sci U S A 102, 5215-5220   DOI
31 Wadia JS and Dowdy SF (2002) Protein transduction technology. Curr Opin Biotechnol 13, 52-56   DOI
32 Jo HS, Eum WS, Park EY et al (2017) Effects of PEP-1-FK506BP on cyst formation in polycystic kidney disease. BMB Rep 50, 460-465   DOI
33 van den Berg A and Dowdy SF (2011) Protein transduction domain delivery of therapeutic macromolecules. Curr Opin Biotechnol 22, 888-893   DOI
34 Kubo E, Fatma N, Akagi Y, Beier DR, Singh SP and Singh DP (2008) TAT-mediated PRDX6 protein transduction protects against eye lens epithelial cell death and delays lens opacity. Am J Physiol Cell Physiol 294, C842-C855   DOI
35 Embury J, Klein D, Pileggi A et al (2001) Proteins linked to a protein transduction domain efficiently transduce pancreatic islets. Diabetes 50, 1706-1713   DOI
36 Jo HS, Yeo HJ, Cha JH et al (2016) Transduced Tat-DJ-1 protein inhibits cytokine-induced pancreatic RINm5F cell death. BMB Rep 49, 297-302   DOI
37 Jo HS, Cha HJ, Kim SJ et al (2016) Tat-DJ-1 inhibits oxidative stress-mediated RINm5F cell death through suppression of $NF-{\kappa}B$ and MAPK activation. Med Chem Res 25, 2589-2598   DOI
38 Jo HS, Yeo EJ, Shin MJ et al (2017) Tat-DJ-1 enhances cell survival by inhibition of oxidative stress, $NF-{\kappa}B$ and MAPK activation in HepG2 cells. Biotechnol Lett 39, 511-521   DOI
39 Kim MJ, Kim DW, Lee BY et al (2013) Transduced Tat-glyoxalase protein attenuates streptozotocin-induced diabetes in a mouse model. Biochem Biophys Res Commun 430, 294-300   DOI
40 Szkudelski T (2001) The mechanisms of alloxan and streptozotocin action in ${\beta}$-cells of rat pancreas. Physiol Res 50, 536-546
41 Jain D, Weber G, Eberhard D et al (2015) DJ-1 protects pancreatic beta cells from cytokine- and streptozotocinmediated cell death. PLoS One 10, e0138535   DOI
42 Singh R, Bhardwaj P and Sharma P (2013) Antioxidant and toxicological evaluation of Cassia sopherain streptozotocin-induced diabetic Wister rats. Pharmacognosy Res 5, 225-232   DOI
43 Robertson RP (2009) Beta-cell deterioration during diabetes: what's in the gun? Trends Endocrinol Metab 20, 388-393   DOI
44 Mota M, Banini BA, Cazanave SC and Sanyal AJ (2016) Molecular mechanism of lipotoxicity and glucotoxicity in nonalcoholic fatty liver disease. Metabolism 65, 1049-1061   DOI
45 Navarro C, Montilla P, Martin A, Jimenez J and Utrilla P (1993) Free radicals scavenger and antihepatotoxic activity of Rosmarinus. Planta Med 59, 312-314   DOI
46 Ren H, Fu K, Mu C, Li B, Wang D and Wang G (2010) DJ-1, a cancer and Parkinson's disease associated protein, regulates autophagy through JNK pathway in cancer cells. Cancer Lett 297, 101-108   DOI
47 Gu L, Cui T, Fan C et al (2009) Involvement of ERK1/2 signaling pathway in DJ-1-induced neuroprotection against oxidative stress. Biochem Biophys Res Commun 383, 469-474   DOI
48 Im JY, Lee KW, Junn E and Mouradian MM (2010) DJ-1 protects against oxidative damage by regulating the thioredoxin/ASK1 complex. Neurosci Res 67, 203-208   DOI
49 Sakai N, Wada T, Furuichi K et al (2002) p38 MAPK phosphorylation and NF-kappa B activation in human crescentic glomerulonephritis. Nephrol Dial Transplant 17, 998-1004   DOI
50 Jaramillo-Gomez J, Nino A, Arboleda H and Arboleda G (2015) Overexpression of DJ-1 protects against C2-ceramide-induced neuronal death through activation of the PI3K/AKT pathway and inhibition of autophagy. Neurosci Lett 603, 71-76   DOI