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http://dx.doi.org/10.5483/BMBRep.2018.51.7.106

Actin-binding LIM protein 1 regulates receptor activator of NF-κB ligand-mediated osteoclast differentiation and motility  

Jin, Su Hyun (Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine)
Kim, Hyunsoo (Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine)
Gu, Dong Ryun (Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine)
Park, Keun Ha (Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine)
Lee, Young Rae (Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine)
Choi, Yongwon (Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine)
Lee, Seoung Hoon (Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine)
Publication Information
BMB Reports / v.51, no.7, 2018 , pp. 356-361 More about this Journal
Abstract
Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of $NF-{\kappa}B$ ligand (RANKL)-mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner. Coincident with the downregulated expression of osteoclast differentiation marker genes, the expression levels of c-Fos and the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), critical transcription factors of osteoclastogenesis, were also decreased in shAblim1-infected osteoclasts during RANKL-mediated osteoclast differentiation. In addition, the motility of preosteoclast was reduced by ABLIM1 knockdown via modulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt/Rac1 signaling pathway, suggesting another regulatory mechanism of ABLIM1 in osteoclast formation. These data demonstrated that ABLIM1 is a positive regulator of RANKL-mediated osteoclast formation via the modulation of the differentiation and PI3K/Akt/Rac1-dependent motility.
Keywords
ABLIM1; LIM domain; Motility; Osteoclast; RANKL;
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1 Huang Q, Shen HM and Ong CN (2005) Emodin inhibits tumor cell migration through suppression of the phosphatidylinositol 3-kinase-Cdc42/Rac1 pathway. Cell Mol Life Sci 62, 1167-1175   DOI
2 Zhang LJ, Tao BB, Wang MJ, Jin HM and Zhu YC (2012) PI3K p110alpha isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganization. PLoS One 7, e44590   DOI
3 Itzstein C, Coxon FP and Rogers MJ (2011) The regulation of osteoclast function and bone resorption by small GTPases. Small GTPases 2, 117-130   DOI
4 Fukuda A, Hikita A, Wakeyama H et al (2005) Regulation of osteoclast apoptosis and motility by small GTPase binding protein Rac1. J Bone Miner Res 20, 2245-2253   DOI
5 Oh SJ, Gu DR, Jin SH, Park KH and Lee SH (2016) Cytosolic malate dehydrogenase regulates RANKL-mediated osteoclastogenesis via AMPK/c-Fos/NFATc1 signaling. Biochem Biophys Res Commun 475, 125-132   DOI
6 Kim I, Kim JH, Kim K, Seong S and Kim N (2017) Tusc2/Fus1 regulates osteoclast differentiation through $NF-{\kappa}B$ and NFATc1. BMB Rep 50, 454-459   DOI
7 Ford CE, Jary E, Ma SS, Nixdorf S, Heinzelmann-Schwarz VA and Ward RL (2013) The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells. PLoS One 8, e54362   DOI
8 Takayanagi H (2007) Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat Rev Immunol 7, 292-304   DOI
9 Walsh MC, Kim N, Kadono Y et al (2006) Osteoimmunology: interplay between the immune system and bone metabolism. Annu Rev Immunol 24, 33-63   DOI
10 Takayanagi H (2007) The role of NFAT in osteoclast formation. Ann N Y Acad Sci 1116, 227-237   DOI
11 Kim K, Kim JH, Lee J et al (2005) Nuclear factor of activated T cells c1 induces osteoclast-associated receptor gene expression during tumor necrosis factor-related activation-induced cytokine-mediated osteoclastogenesis. J Biol Chem 280, 35209-35216   DOI
12 Baroukh B, Cherruau M, Dobigny C, Guez D and Saffar JL (2000) Osteoclasts differentiate from resident precursors in an in vivo model of synchronized resorption: a temporal and spatial study in rats. Bone 27, 627-634   DOI
13 Roodman GD (1996) Advances in bone biology: the osteoclast. Endocr Rev 17, 308-332
14 Bach I (2000) The LIM domain: regulation by association. Mech Dev 91, 5-17   DOI
15 Boudot C, Saidak Z, Boulanouar AK et al (2010) Implication of the calcium sensing receptor and the Phosphoinositide 3-kinase/Akt pathway in the extracellular calcium-mediated migration of RAW 264.7 osteoclast precursor cells. Bone 46, 1416-1423   DOI
16 Kim JM, Kim MY, Lee K and Jeong D (2016) Distinctive and selective route of PI3K/PKCalpha-PKCdelta/RhoA-Rac1 signaling in osteoclastic cell migration. Mol Cell Endocrinol 437, 261-267   DOI
17 Lee NK, Choi HK, Kim DK and Lee SY (2006) Rac1 GTPase regulates osteoclast differentiation through TRANCE-induced NF-kappa B activation. Mol Cell Biochem 281, 55-61   DOI
18 Wang Y, Lebowitz D, Sun C, Thang H, Grynpas MD and Glogauer M (2008) Identifying the relative contributions of Rac1 and Rac2 to osteoclastogenesis. J Bone Miner Res 23, 260-270
19 Schmeichel KL and Beckerle MC (1994) The LIM domain is a modular protein-binding interface. Cell 79, 211-219   DOI
20 Koch BJ, Ryan JF and Baxevanis AD (2012) The diversification of the LIM superclass at the base of the metazoa increased subcellular complexity and promoted multicellular specialization. PLoS One 7, e33261   DOI
21 Roof DJ, Hayes A, Adamian M, Chishti AH and Li T (1997) Molecular characterization of abLIM, a novel actin-binding and double zinc finger protein. J Cell Biol 138, 575-588   DOI
22 Barrientos T, Frank D, Kuwahara K et al (2007) Two novel members of the ABLIM protein family, ABLIM-2 and -3, associate with STARS and directly bind F-actin. J Biol Chem 282, 8393-8403   DOI
23 Muller JM, Metzger E, Greschik H et al (2002) The transcriptional coactivator FHL2 transmits Rho signals from the cell membrane into the nucleus. EMBO J 21, 736-748   DOI
24 Matsuda M, Yamashita JK, Tsukita S and Furuse M (2010) abLIM3 is a novel component of adherens junctions with actin-binding activity. Eur J Cell Biol 89, 807-816   DOI
25 Lu C, Huang X, Ma HF et al (2003) Normal retinal development and retinofugal projections in mice lacking the retina-specific variant of actin-binding LIM domain protein. Neuroscience 120, 121-131   DOI
26 Kadrmas JL and Beckerle MC (2004) The LIM domain: from the cytoskeleton to the nucleus. Nat Rev Mol Cell Biol 5, 920-931   DOI
27 Pawson T and Nash P (2003) Assembly of cell regulatory systems through protein interaction domains. Science 300, 445-452   DOI
28 Zheng Q and Zhao Y (2007) The diverse biofunctions of LIM domain proteins: determined by subcellular localization and protein-protein interaction. Biol Cell 99, 489-502   DOI
29 Chang DF, Belaguli NS, Iyer D et al (2003) Cysteine-rich LIM-only proteins CRP1 and CRP2 are potent smooth muscle differentiation cofactors. Dev Cell 4, 107-118   DOI
30 Feng Y, Zhao H, Luderer HF et al (2007) The LIM protein, Limd1, regulates AP-1 activation through an interaction with Traf6 to influence osteoclast development. J Biol Chem 282, 39-48   DOI
31 Struckhoff EC and Lundquist EA (2003) The actin-binding protein UNC-115 is an effector of Rac signaling during axon pathfinding in C. elegans. Development 130, 693-704   DOI