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http://dx.doi.org/10.5483/BMBRep.2018.51.5.198

Selective regulation of osteoclast adhesion and spreading by PLCγ/PKCα-PKCδ/RhoA-Rac1 signaling  

Kim, Jin-Man (Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine)
Lee, Kyunghee (Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine)
Jeong, Daewon (Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine)
Publication Information
BMB Reports / v.51, no.5, 2018 , pp. 230-235 More about this Journal
Abstract
Bone resorption by multinucleated osteoclasts is a multistep process involving adhesion to the bone matrix, migration to resorption sites, and formation of sealing zones and ruffled borders. Macrophage colony-stimulating factor (M-CSF) and osteopontin (OPN) have been shown to be involved in the bone resorption process by respective activation of integrin ${\alpha}v{\beta}3$ via "inside-out" and "outside-in" signaling. In this study, we investigated the link between signal modulators known to M-CSF- and OPN-induced osteoclast adhesion and spreading. M-CSF- and OPN-induced osteoclast adhesion was achieved via activation of stepwise signals, including integrin ${\alpha}v{\beta}3$, $PLC{\gamma}$, $PKC{\delta}$, and Rac1. Osteoclast spreading induced by M-CSF and OPN was shown to be controlled via sequential activation, consistent with the osteoclast adhesion processes. In contrast to osteoclast adhesion, osteoclast spreading induced by M-CSF and OPN was blocked via activation of $PLC{\gamma}/PKC{\alpha}/RhoA$ signaling. The combined results indicate that osteoclast adhesion and spreading are selectively regulated via $PLC{\gamma}/PKC{\alpha}-PKC{\delta}/RhoA-Rac1$ signaling.
Keywords
Integrin ${\alpha}v{\beta}3$; Osteoclast adhesion; Osteoclast spreading; Phospholipase C; Protein kinase C;
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