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http://dx.doi.org/10.5483/BMBRep.2017.50.8.103

SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion  

Moon, Heegyum (School of Life Sciences, Gwangju Institute of Science and Technology)
Cho, Sunghee (School of Life Sciences, Gwangju Institute of Science and Technology)
Loh, Tiing Jen (School of Life Sciences, Gwangju Institute of Science and Technology)
Jang, Ha Na (School of Life Sciences, Gwangju Institute of Science and Technology)
Liu, Yongchao (School of Life Sciences, Gwangju Institute of Science and Technology)
Choi, Namjeong (School of Life Sciences, Gwangju Institute of Science and Technology)
Oh, Jagyeong (School of Life Sciences, Gwangju Institute of Science and Technology)
Ha, Jiyeon (School of Life Sciences, Gwangju Institute of Science and Technology)
Zhou, Jianhua (JiangSu Key Laboratory of Neuroregeneration, Nantong University)
Cho, Sungchan (Bio-Therapeutics Research Institute, Korea Research Institute of Bioscience and Biotechnology)
Kim, Dong-Eun (Department of Bioscience and Biotechnology, Konkuk University)
Ye, Michael B. (Division of Liberal Arts and Sciences, Gwangju Institute of Science and Technology)
Zheng, Xuexiu (School of Life Sciences, Gwangju Institute of Science and Technology)
Shen, Haihong (School of Life Sciences, Gwangju Institute of Science and Technology)
Publication Information
BMB Reports / v.50, no.8, 2017 , pp. 423-428 More about this Journal
Abstract
SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN pre-mRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.
Keywords
Exon exclusion; Intron retention; Pre-mRNA splicing; SRSF2;
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Times Cited By KSCI : 2  (Citation Analysis)
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