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http://dx.doi.org/10.5483/BMBRep.2017.50.6.217

Identifying the cellular location of brain cytoplasmic 200 RNA using an RNA-recognizing antibody  

Shin, Heegwon (Department of Chemistry, KAIST)
Lee, Jungmin (Department of Chemistry, KAIST)
Kim, Youngmi (Department of Chemistry, KAIST)
Jang, Seonghui (Department of Chemistry, KAIST)
Ohn, Takbum (Department of Cellular and Molecular Medicine, Chosun University School of Medicine)
Lee, Younghoon (Department of Chemistry, KAIST)
Publication Information
BMB Reports / v.50, no.6, 2017 , pp. 318-322 More about this Journal
Abstract
Brain cytoplasmic 200 RNA (BC200 RNA) is a neuron-specific non-coding RNA, implicated in the inhibition of local synaptodendritic protein synthesis, and is highly expressed in some cancer cells. Although BC200 RNA has been shown to inhibit translation in vitro, the cellular location of this inhibition is unknown. In this study, we used a BC200 RNA-recognizing antibody to identify the cellular locations of BC200 RNA in HeLa cervical carcinoma cells. We observed punctate signals in both the cytoplasm and nucleus, and further discovered that BC200 RNA co-localized with the p-body decapping enzyme, DCP1A, and the heterogeneous nuclear ribonucleoprotein E2 (hnRNP E2). The latter is a known BC200 RNA-binding partner protein and a constituent of p-bodies. This suggests that BC200 RNA is localized to p-bodies via hnRNP E2.
Keywords
BC200 RNA; hnRNP E2; p-body; RNA-recognizing antibody;
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Times Cited By KSCI : 2  (Citation Analysis)
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