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http://dx.doi.org/10.5483/BMBRep.2017.50.12.158

Gastrin-releasing peptide promotes the migration of vascular smooth muscle cells through upregulation of matrix metalloproteinase-2 and -9  

Park, Hyun-Joo (Department of Oral Physiology, BK21 PLUS Project, School of Dentistry, Pusan National University)
Kim, Mi-Kyoung (Department of Oral Physiology, BK21 PLUS Project, School of Dentistry, Pusan National University)
Kim, Yeon (Department of Oral Physiology, BK21 PLUS Project, School of Dentistry, Pusan National University)
Bae, Sun Sik (Department of Pharmacology, Pusan National University School of Medicine, Pusan National University)
Kim, Hyung Joon (Department of Oral Physiology, BK21 PLUS Project, School of Dentistry, Pusan National University)
Bae, Soo-Kyung (Department of Dental Pharmacology, BK21 PLUS Project, School of Dentistry, Pusan National University)
Bae, Moon-Kyoung (Department of Oral Physiology, BK21 PLUS Project, School of Dentistry, Pusan National University)
Publication Information
BMB Reports / v.50, no.12, 2017 , pp. 628-633 More about this Journal
Abstract
Gastrin-releasing peptide (GRP) has been reported to be implicated in the pathogenesis of inflammatory disorders. The migration and proliferation of vascular smooth muscle cells (VSMCs) are key components of vascular inflammation that leads to the development of atherosclerosis. The present study aimed to investigate the molecular effect of GRP on VSMC proliferation and migration. We report that GRP significantly enhanced the proliferation and migration of rat VSMCs. GRP increased mRNA and protein expression of matrix metalloproteinase-2 and -9 (MMP-2/9) in VSMCs. The induction of MMP-2/9 by GRP was regulated by the activation of the signal transducer and activator of transcription-3 (STAT3). In addition, STAT3-knockdown of VSMCs by siRNA or blockade of the GRP receptor inhibited GRP-induced migration of VSMCs. Taken together, our findings indicate that GRP promotes the migration of VSMCs through upregulation of MMP-2/9 via STAT3 activation.
Keywords
Gastrin-releasing peptide; Matrix metalloproteinase-2/9; Migration; STAT-3; Vascular smooth muscle cells;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Majumdar ID and Weber HC (2011) Biology of mammalian bombesin-like peptides and their receptors. Curr Opin Endocrinol Diabetes Obes 18, 68-74   DOI
2 Gonzalez N, Moody TW, Igarashi H et al (2008) Bombesin-related peptides and their receptors: Recent advances in their role in physiology and disease states. Curr Opin Endocrinol Diabetes Obes 15, 58-64   DOI
3 Roesler R, Kent P, Luft T et al (2014) Gastrin-releasing peptide receptor signaling in the integration of stress and memory. Neurobiol Learn Mem 112, 44-52   DOI
4 Rao RM, Yang L, Garcia-Cardena G et al (2007) Endothelial-dependent mechanisms of leukocyte recruitment to the vascular wall. Circ Res 101, 234-247   DOI
5 Sprague AH and Khalil RA (2009) Inflammatory cytokines in vascular dysfunction and vascular disease. Biochem Pharmacol 78, 539-552   DOI
6 Czepielewski RS, Porto BN, Rizzo LB et al (2012) Gastrinreleasing peptide receptor (GRPR) mediates chemotaxis in neutrophils. Proc Natl Acad Sci U S A 109, 547-552   DOI
7 Kim MK, Park HJ, Kim Y et al (2017) Gastrin-releasing peptide induces monocyte adhesion to vascular endothelium by upregulating endothelial adhesion molecules. Biochem Biophys Res Commun 485, 542-549   DOI
8 Owens GK, Kumar MS and Wamhoff BR (2004) Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev 84, 767-801   DOI
9 Abedi H and Zachary I (1995) Signalling mechanisms in the regulation of vascular cell migration. Cardiovasc Res 30, 544-556   DOI
10 Newby AC (2006) Matrix metalloproteinases regulate migration, proliferation, and death of vascular smooth muscle cells by degrading matrix and non-matrix substrates. Cardiovasc Res 69, 614-624   DOI
11 Doran AC, Meller N and McNamara CA (2008) Role of smooth muscle cells in the initiation and early progression of atherosclerosis. Arterioscler Thromb Vasc Biol 28, 812-819   DOI
12 Sancho V, Di Florio A, Moody TW et al (2011) Bombesin receptor-mediated imaging and cytotoxicity: Review and current status. Curr Drug Deliv 8, 79-134   DOI
13 Oliveira PG, Grespan R, Pinto LG et al (2011) Protective effect of RC-3095, an antagonist of the gastrin-releasing peptide receptor, in experimental arthritis. Arthritis Rheum 63, 2956-2965   DOI
14 Ghosh A, Pechota A, Coleman D et al (2015) Cigarette smoke-induced MMP2 and MMP9 secretion from aortic vascular smooth cells is mediated via the Jak/Stat pathway. Hum Pathol 46, 284-294   DOI
15 Seki Y, Kai H, Shibata R et al (2000) Role of the JAK/STAT pathway in rat carotid artery remodeling after vascular injury. Circ Res 87, 12-18   DOI
16 Daniel JM, Dutzmann J, Bielenberg W et al (2012) Inhibition of STAT3 signaling prevents vascular smooth muscle cell proliferation and neointima formation. Basic Res Cardiol 107, 261-272   DOI
17 Willis AI, Pierre-Paul D, Sumpio BE et al (2004) Vascular smooth muscle cell migration: Current research and clinical implications. Vasc Endovascular Surg 38, 11-23   DOI
18 Dzau VJ, Braun-Dullaeus RC and Sedding DG (2002) Vascular proliferation and atherosclerosis: New perspectives and therapeutic strategies. Nat Med 8, 1249-1256   DOI
19 Yu H, Lee H, Herrmann A et al (2014) Revisiting STAT3 signalling in cancer: New and unexpected biological functions. Nat Rev Cancer 14, 736-746   DOI
20 Liao XH, Wang N, Zhao DW et al (2015) STAT3 protein regulates vascular smooth muscle cell phenotypic switch by interaction with myocardin. J Biol Chem 290, 19641-19652   DOI
21 Mason DP, Kenagy RD, Hasenstab D et al (1999) Matrix metalloproteinase-9 overexpression enhances vascular smooth muscle cell migration and alters remodeling in the injured rat carotid artery. Circ Res 85, 1179-1185   DOI
22 Dutzmann J, Daniel JM, Bauersachs J et al (2015) Emerging translational approaches to target STAT3 signalling and its impact on vascular disease. Cardiovasc Res 106, 365-374   DOI
23 Caird J, Napoli C, Taggart C et al (2006) Matrix metalloproteinases 2 and 9 in human atherosclerotic and nonatherosclerotic cerebral aneurysms. Eur J Neurol 13, 1098-1105   DOI
24 Wagsater D, Zhu C, Bjorkegren, J et al (2011) MMP-2 and MMP-9 are prominent matrix metalloproteinases during atherosclerosis development in the Ldlr(-/-)Apob(100/100) mouse. Int J Mol Med 28, 247-253
25 Belo VA, Guimaraes D and Castro MM (2015) Matrix Metalloproteinase 2 as a Potential Mediator of Vascular Smooth Muscle Cell Migration and Chronic Vascular Remodeling in Hypertension. J Vasc Res 52, 221-231   DOI
26 Vincenti MP and Brinckerhoff CE (2007) Signal transduction and cell-type specific regulation of matrix metalloproteinase gene expression: Can MMPs be good for you? J Cell Physiol 213, 355-364   DOI
27 Sun HJ, Zhao MX, Ren XS et al (2016) Salusin-beta promotes vascular smooth muscle cell migration and intimal hyperplasia after vascular injury via ROS/NFkappaB/MMP-9 pathway. Antioxid Redox Signal 24, 1045-1057   DOI
28 Fan Y, Mao R and Yang J (2013) NF-kappaB and STAT3 signaling pathways collaboratively link inflammation to cancer. Protein Cell 4, 176-185   DOI
29 Dong DD, Zhou H and Li G (2016) GPR78 promotes lung cancer cell migration and metastasis by activation of Gaq-Rho GTPase pathway. BMB Rep 49, 623-628   DOI
30 Choi SH, Park BK, Lee KW et al (2015) Effect of respiratory syncytial virus on the growth of hepatocellular carcinoma cell-lines. BMB Rep 48, 565-570   DOI