Browse > Article
http://dx.doi.org/10.5483/BMBRep.2017.50.12.096

Fibronectin expression is upregulated by PI-3K/Akt activation in tamoxifen-resistant breast cancer cells  

You, Daeun (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University)
Jung, Seung Pil (Division of Breast and Endocrine Surgery, Department of Surgery, Korea University Hospital, Korea University College of Medicine)
Jeong, Yisun (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University)
Bae, Soo Youn (Division of Breast and Endocrine Surgery, Department of Surgery, Korea University Hospital, Korea University College of Medicine)
Lee, Jeong Eon (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University)
Kim, Sangmin (Breast Cancer Center, Samsung Medical Center)
Publication Information
BMB Reports / v.50, no.12, 2017 , pp. 615-620 More about this Journal
Abstract
Fibronectin (FN) plays important roles in the EMT in a variety of cancer cell types. However, the mechanism by which FN expression is regulated in tamoxifen-resistant (TamR) breast cancer cells has not yet been fully elucidated. Aberrant FN expression was associated with poor prognosis in patients with luminal type A breast cancer. In addition, FN was upregulated in TamR cells. To investigate the mechanism by which FN expression is regulated, we assessed the levels of phosphorylated Akt, JNK, and STAT3 and found that they were all increased in TamR cells. Induction of FN expression was dampened by LY294002 or AKT IV in TamR cells. Furthermore, FN expression was increased by constitutively active (CA)-Akt overexpression in tamoxifen-sensitive MCF7 (TamS) cells and colony formation of TamR cells was blocked by AKT IV treatment. Taken together, these results demonstrate that FN expression is upregulated through the PI-3K/Akt pathway in tamoxifen-resistant breast cancer cells.
Keywords
Akt pathway; Fibronectin; Poor prognosis; Tamoxifen resistance;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Beiki O, Hall P, Ekbom A and Moradi T (2012) Breast cancer incidence and case fatality among 4.7 million women in relation to social and ethnic background: a population-based cohort study. Breast Cancer Res 14, R5   DOI
2 Lim E, Metzger-Filho O and Winer EP (2012) The natural history of hormone receptor-positive breast cancer. Oncology (Williston Park) 26, 688-694, 696
3 Regan MM, Neven P, Giobbie-Hurder A et al (2011) Assessment of letrozole and tamoxifen alone and in sequence for postmenopausal women with steroid hormone receptor-positive breast cancer: the BIG 1-98 randomised clinical trial at 8.1 years median follow-up. Lancet Oncol 12, 1101-1108   DOI
4 Colditz GA (1998) Relationship between estrogen levels, use of hormone replacement therapy, and breast cancer. J Natl Cancer Inst 90, 814-823   DOI
5 Paridaens R, Sylvester RJ, Ferrazzi E, Legros N, Leclercq G and Heuson JC (1980) Clinical significance of the quantitative assessment of estrogen receptors in advanced breast cancer. Cancer 46, 2889-2895   DOI
6 Ravdin PM, Fritz NF, Tormey DC and Jordan VC (1988) Endocrine status of premenopausal node-positive breast cancer patients following adjuvant chemotherapy and long-term tamoxifen. Cancer Res 48, 1026-1029
7 Anzick SL, Kononen J, Walker RL et al (1997) AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science 277, 965-968   DOI
8 Ruoslahti E (1988) Fibronectin and its receptors. Annu Rev Biochem 57, 375-413   DOI
9 Jeon M, Lee J, Nam SJ, Shin I, Lee JE and Kim S (2015) Induction of fibronectin by HER2 overexpression triggers adhesion and invasion of breast cancer cells. Exp Cell Res 333, 116-126   DOI
10 Kim S, Jeon M, Lee J et al (2014) Induction of fibronectin in response to epidermal growth factor is suppressed by silibinin through the inhibition of STAT3 in triple negative breast cancer cells. Oncol Rep 32, 2230-2236   DOI
11 Dean DC, Newby RF and Bourgeois S (1988) Regulation of fibronectin biosynthesis by dexamethasone, transforming growth factor beta, and cAMP in human cell lines. J Cell Biol 106, 2159-2170   DOI
12 Blatti SP, Foster DN, Ranganathan G, Moses HL and Getz MJ (1988) Induction of fibronectin gene transcription and mRNA is a primary response to growth-factor stimulation of AKR-2B cells. Proc Natl Acad Sci U S A 85, 1119-1123   DOI
13 McDonald JA (1988) Extracellular matrix assembly. Annu Rev Cell Biol 4, 183-207   DOI
14 Dean DC (1989) Expression of the fibronectin gene. Am J Respir Cell Mol Biol 1, 5-10   DOI
15 Bae YK, Kim A, Kim MK, Choi JE, Kang SH and Lee SJ (2013) Fibronectin expression in carcinoma cells correlates with tumor aggressiveness and poor clinical outcome in patients with invasive breast cancer. Hum Pathol 44, 2028-2037   DOI
16 Kim S, Lee J, Oh SJ, Nam SJ and Lee JE (2015) Differential effect of EGFR inhibitors on tamoxifen-resistant breast cancer cells. Oncol Rep 34, 1613-1619   DOI
17 Ali S and Coombes RC (2002) Endocrine-responsive breast cancer and strategies for combating resistance. Nat Rev Cancer 2, 101-112   DOI
18 Arpino G, Green SJ, Allred DC et al (2004) HER-2 amplification, HER-1 expression, and tamoxifen response in estrogen receptor-positive metastatic breast cancer: a southwest oncology group study. Clin Cancer Res 10, 5670-5676   DOI
19 Ioachim E, Charchanti A, Briasoulis E et al (2002) Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression. Eur J Cancer 38, 2362-2370   DOI
20 Raimondi C, Gradilone A, Naso G et al (2011) Epithelialmesenchymal transition and stemness features in circulating tumor cells from breast cancer patients. Breast Cancer Res Treat 130, 449-455   DOI
21 Jaiswal BS, Janakiraman V, Kljavin NM et al (2009) Somatic mutations in p85alpha promote tumorigenesis through class IA PI3K activation. Cancer Cell 16, 463-474   DOI
22 Oh S, Kim H, Nam K, Shin I (2017) Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells. BMB Rep 50, 132-137   DOI
23 Lee BH, Kim MS, Rhew JH, Park RW, de Crombrugghe B and Kim IS (2000) Transcriptional regulation of fibronectin gene by phorbol myristate acetate in hepatoma cells: a negative role for NF-kappaB. J Cell Biochem 76, 437-451   DOI
24 Chen S, Mukherjee S, Chakraborty C and Chakrabarti S (2003) High glucose-induced, endothelin-dependent fibronectin synthesis is mediated via NF-kappa B and AP-1. Am J Physiol Cell Physiol 284, C263-272   DOI
25 Campbell IG, Russell SE, Choong DY et al (2004) Mutation of the PIK3CA gene in ovarian and breast cancer. Cancer Res 64, 7678-7681   DOI
26 Park S, Sim H, Lee K (2016) Rapamycin-resistant and torin-sensitive mTOR signaling promotes the survival and proliferation of leukemic cells. BMB Rep 49, 63-68   DOI
27 Zheng LH, Zhao YH, Feng HL and Liu YJ (2014) Endocrine resistance in breast cancer. Climacteric 17, 522-528   DOI
28 Perez-Tenorio G, Alkhori L, Olsson B et al (2007) PIK3CA mutations and PTEN loss correlate with similar prognostic factors and are not mutually exclusive in breast cancer. Clin Cancer Res 13, 3577-3584   DOI
29 Fedele CG, Ooms LM, Ho M et al (2010) Inositol polyphosphate 4-phosphatase II regulates PI3K/Akt signaling and is lost in human basal-like breast cancers. Proc Natl Acad Sci U S A 107, 22231-22236   DOI
30 Miller TW, Balko JM and Arteaga CL (2011) Phosphatidylinositol 3-kinase and antiestrogen resistance in breast cancer. J Clin Oncol 29, 4452-4461   DOI
31 Choi HK, Yang JW, Roh SH, Han CY and Kang KW (2007) Induction of multidrug resistance associated protein 2 in tamoxifen-resistant breast cancer cells. Endocr Relat Cancer 14, 293-303   DOI
32 Gyorffy B, Lanczky A, Eklund AC et al (2010) An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res Treat 123, 725-731   DOI