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http://dx.doi.org/10.5483/BMBRep.2017.50.11.060

Cytoprotective effect exerted by geraniin in HepG2 cells is through microRNA mediated regulation of BACH-1 and HO-1  

Aayadi, Hoda (Department of Zoology, Savitribai Phule Pune University)
Mittal, Smriti P.K. (Department of Zoology, Savitribai Phule Pune University)
Deshpande, Anjali (AJ Organica Pvt. Ltd.)
Gore, Makarand (AJ Organica Pvt. Ltd.)
Ghaskadbi, Saroj S. (Department of Zoology, Savitribai Phule Pune University)
Publication Information
BMB Reports / v.50, no.11, 2017 , pp. 560-565 More about this Journal
Abstract
Geraniin, a hydrolysable tannin, used in traditional medicine in Southeast Asia, is known to exhibit various biological activities. As an antioxidant it is known to up-regulate phase II enzyme Heme oxygenase-1 (HO-1). However its mechanism is not clearly understood. Nuclear factor erythroid-derived 2 related factor 2 (Nrf-2) is transcriptionally up-regulated by Extracellular signal-regulated kinase (ERK) 1/2 and retained in nucleus due to inactivated Glycogen synthase kinase 3 beta ($GSK-3{\beta}$). Geraniin additionally down-regulates expression of microRNA 217 and 377 (miR-217 and miR-377) which target HO-1 mRNA. Expression of BTB and CNC homolog 1 (BACH-1), another regulator of HO-1, is also down-regulated by up-regulating microRNA 98 (miR-98), a negative regulator of BACH-1. Thus, geraniin up-regulates HO-1 expression both through activating its positive regulator Nrf-2 and by down-regulating its negative regulator BACH-1. Up-regulation of HO-1 also confers protection to HepG2 cells from tertiary butyl hydroperoxide (TBH) induced cytotoxicity.
Keywords
Antioxidants; Geraniin; HO-1; miRNAs; Nrf-2;
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