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http://dx.doi.org/10.5483/BMBRep.2016.49.9.098

Promotion of formyl peptide receptor 1-mediated neutrophil chemotactic migration by antimicrobial peptides isolated from the centipede Scolopendra subspinipes mutilans  

Park, Yoo Jung (Department of Biological Sciences, Sungkyunkwan University)
Lee, Sung Kyun (Department of Biological Sciences, Sungkyunkwan University)
Jung, Young Su (Department of Biological Sciences, Sungkyunkwan University)
Lee, Mingyu (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University)
Lee, Ha Young (Department of Biological Sciences, Sungkyunkwan University)
Kim, Sang Doo (Department of Biological Sciences, Sungkyunkwan University)
Park, Joon Seong (Department of Hematology-Oncology, Ajou University School of Medicine)
Koo, JaeHyung (Department of Brain and Cognitive Sciences, DGIST)
Hwang, Jae Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Bae, Yoe-Sik (Department of Biological Sciences, Sungkyunkwan University)
Publication Information
BMB Reports / v.49, no.9, 2016 , pp. 520-525 More about this Journal
Abstract
We investigated the effects of two antimicrobial peptides (AMPs) isolated from Scolopendra subspinipes mutilans on neutrophil activity. Stimulation of mouse neutrophils with the two AMPs elicited chemotactic migration of the cells in a pertussis toxin-sensitive manner. The two AMPs also stimulated activation of ERK and Akt, which contribute to chemotactic migration of neutrophils. We found that AMP-stimulated neutrophil chemotaxis was blocked by a formyl peptide receptor (FPR) 1 antagonist (cyclosporin H); moreover the two AMPs stimulated the chemotactic migration of FPR1-expressing RBL-2H3 cells but not of vector-expressing RBL-2H3 cells. We also found that the two AMPs stimulate neutrophil migration in vivo, and that this effect is blocked in FPR1-deficient mice. Taken together, our results suggest that the two AMPs stimulate neutrophils, leading to chemotactic migration through FPR1, and the two AMPs will be useful for the study of FPR1 signaling and neutrophil activation.
Keywords
Antimicrobial peptide; Chemotaxis; Formyl peptide receptor 1; Neutrophil; Scolopendra subspinipes mutilans;
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Times Cited By KSCI : 3  (Citation Analysis)
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