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http://dx.doi.org/10.5483/BMBRep.2016.49.8.020

Critical role of protein L-isoaspartyl methyltransferase in basic fibroblast growth factor-mediated neuronal cell differentiation  

Dung, To Thi Mai (Department of Genetic Engineering, Sungkyunkwan University)
Yi, Young-Su (Department of Genetic Engineering, Sungkyunkwan University)
Heo, Jieun (Department of Genetic Engineering, Sungkyunkwan University)
Yang, Woo Seok (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Ji Hye (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Han Gyung (Department of Genetic Engineering, Sungkyunkwan University)
Park, Jae Gwang (Department of Genetic Engineering, Sungkyunkwan University)
Yoo, Byong Chul (Colorectal Cancer Branch, Research Institute, National Cancer Center)
Cho, Jae Youl (Department of Genetic Engineering, Sungkyunkwan University)
Hong, Sungyoul (Department of Genetic Engineering, Sungkyunkwan University)
Publication Information
BMB Reports / v.49, no.8, 2016 , pp. 437-442 More about this Journal
Abstract
We aimed to study the role of protein L-isoaspartyl methyltransferase (PIMT) in neuronal differentiation using basic fibroblast growth factor (bFGF)-induced neuronal differentiation, characterized by cell-body shrinkage, long neurite outgrowth, and expression of neuronal differentiation markers light and medium neurofilaments (NF). The bFGF-mediated neuronal differentiation of PC12 cells was induced through activation of mitogen-activated protein kinase (MAPK) signaling molecules [MAPK kinase 1/2 (MEK1/2), extracellular signal-regulated kinase 1/2 (ERK1/2), and p90RSK], and phosphatidylinositide 3-kinase (PI3K)/Akt signaling molecules PI3Kp110β, PI3Kp110γ, Akt, and mTOR. Inhibitors (adenosine dialdehyde and S-adenosylhomocysteine) of protein methylation suppressed bFGF-mediated neuronal differentiation of PC12 cells. PIMT-eficiency caused by PIMT-specific siRNA inhibited neuronal differentiation of PC12 cells by suppressing phosphorylation of MEK1/2 and ERK1/2 in the MAPK signaling pathway and Akt and mTOR in the PI3K/Akt signaling pathway. Therefore, these results suggested that PIMT was critical for bFGF-mediated neuronal differentiation of PC12 cells and regulated the MAPK and Akt signaling pathways.
Keywords
Akt; Basic fibroblast growth factor; Mitogen-activated protein kinase; Neuronal differentiation; Phosphatidylinositide 3-kinase; Protein L-isoaspartyl methyltransferase;
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1 Ma Q (2006) Transcriptional regulation of neuronal phenotype in mammals. J Physiol 575, 379-387   DOI
2 Feng Z and Porter AG (1999) NF-kappaB/Rel proteins are required for neuronal differentiation of SH-SY5Y neuroblastoma cells. J Biol Chem 274, 30341-30344   DOI
3 Leppa S, Eriksson M, Saffrich R, Ansorge W and Bohmann D (2001) Complex functions of AP-1 transcription factors in differentiation and survival of PC12 cells. Mol Cell Biol 21, 4369-4378   DOI
4 Woodbury D, Schwarz EJ, Prockop DJ and Black IB (2000) Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 61, 364-370   DOI
5 Doniach T (1995) Basic FGF as an inducer of anteroposterior neural pattern. Cell 83, 1067-1070   DOI
6 Chiba S, Kurokawa MS, Yoshikawa H et al (2005) Noggin and basic FGF were implicated in forebrain fate and caudal fate, respectively, of the neural tube-like structures emerging in mouse ES cell culture. Exp Brain Res 163, 86-99   DOI
7 Stemple DL, Mahanthappa NK and Anderson DJ (1988) Basic FGF induces neuronal differentiation, cell division, and NGF dependence in chromaffin cells: a sequence of events in sympathetic development. Neuron 1, 517-525   DOI
8 Yang H, Xia Y, Lu SQ, Soong TW and Feng ZW (2008) Basic fibroblast growth factor-induced neuronal differentiation of mouse bone marrow stromal cells requires FGFR-1, MAPK/ERK, and transcription factor AP-1. J Biol Chem 283, 5287-5295   DOI
9 Pittack C, Jones M and Reh TA (1991) Basic fibroblast growth factor induces retinal pigment epithelium to generate neural retina in vitro. Development 113, 577-588
10 Galy A, Neron B, Planque N, Saule S and Eychene A (2002) Activated MAPK/ERK kinase (MEK-1) induces transdifferentiation of pigmented epithelium into neural retina. Dev Biol 248, 251-264   DOI
11 Eswarakumar VP, Lax I and Schlessinger J (2005) Cellular signaling by fibroblast growth factor receptors. Cytokine Growth Factor Rev 16, 139-149   DOI
12 Wang JK, Gao G and Goldfarb M (1994) Fibroblast growth factor receptors have different signaling and mitogenic potentials. Mol Cell Biol 14, 181-188   DOI
13 Street JT, Wang JH, Power CJ and Redmond HP (2000) Priming of neutrophil [Ca2+]1 signaling and oxidative burst by human fracture fluids. J Trauma 49, 167-168   DOI
14 Abe K and Saito H (2000) Neurotrophic effect of basic fibroblast growth factor is mediated by the p42/p44 mitogen-activated protein kinase cascade in cultured rat cortical neurons. Brain Res Dev Brain Res 122, 81-85   DOI
15 Kim HJ, Kim J, Kang KS, Lee KT and Yang HO (2014) Neuroprotective Effect of Chebulagic Acid via Autophagy Induction in SH-SY5Y Cells. Biomol Ther (Seoul) 22, 275-281   DOI
16 Jeon CY, Kim HJ, Morii H et al (2010) Neurite outgrowth from PC12 cells by basic fibroblast growth factor (bFGF) is mediated by RhoA inactivation through p190RhoGAP and ARAP3. J Cell Physiol 224, 786-794   DOI
17 Clarke S (2003) Aging as war between chemical and biochemical processes: protein methylation and the recognition of age-damaged proteins for repair. Ageing Res Rev 2, 263-285   DOI
18 Weintraub SJ and Deverman BE (2007) Chronoregulation by asparagine deamidation. Sci STKE 2007, re7   DOI
19 Reissner KJ and Aswad DW (2003) Deamidation and isoaspartate formation in proteins: unwanted alterations or surreptitious signals? Cell Mol Life Sci 60, 1281-1295   DOI
20 Desrosiers RR and Fanelus I (2011) Damaged proteins bearing L-isoaspartyl residues and aging: a dynamic equilibrium between generation of isomerized forms and repair by PIMT. Curr Aging Sci 4, 8-18   DOI
21 Curnis F, Cattaneo A, Longhi R et al (2010) Critical role of flanking residues in NGR-to-isoDGR transition and CD13/integrin receptor switching. J Biol Chem 285, 9114-9123   DOI
22 Corti A and Curnis F (2011) Isoaspartate-dependent molecular switches for integrin-ligand recognition. J Cell Sci 124, 515-522   DOI
23 Deverman BE, Cook BL, Manson SR et al (2002) Bcl-xL deamidation is a critical switch in the regulation of the response to DNA damage. Cell 111, 51-62   DOI
24 Zhao R, Oxley D, Smith TS et al (2007) DNA damage-induced Bcl-xL deamidation is mediated by NHE-1 antiport regulated intracellular pH. PLoS Biol 5, e1   DOI
25 Zhao R, Yang FT and Alexander DR (2004) An oncogenic tyrosine kinase inhibits DNA repair and DNA-damage-induced Bcl-xL deamidation in T cell transformation. Cancer Cell 5, 37-49   DOI
26 Zhao R, Follows GA, Beer PA et al (2008) Inhibition of the Bcl-xL deamidation pathway in myeloproliferative disorders. N Engl J Med 359, 2778-2789   DOI
27 Bidinosti M, Ran I, Sanchez-Carbente MR et al (2010) Postnatal deamidation of 4E-BP2 in brain enhances its association with raptor and alters kinetics of excitatory synaptic transmission. Mol Cell 37, 797-808   DOI
28 Chavous DA, Jackson FR and O’Connor CM (2001) Extension of the Drosophila lifespan by overexpression of a protein repair methyltransferase. Proc Natl Acad Sci U S A 98, 14814-14818   DOI
29 Khare S, Linster CL and Clarke SG (2011) The interplay between protein L-isoaspartyl methyltransferase activity and insulin-like signaling to extend lifespan in Caenorhabditis elegans. PLoS One 6, e20850   DOI
30 Chittka A (2013) Differential regulation of SC1/PRDM4 and PRMT5 mediated protein arginine methylation by the nerve growth factor and the epidermal growth factor in PC12 cells. Neurosci Lett 550, 87-92   DOI
31 Lin YL, Tsai YJ, Liu YF et al (2013) The critical role of protein arginine methyltransferase prmt8 in zebrafish embryonic and neural development is non-redundant with its paralogue prmt1. PLoS One 8, e55221   DOI
32 Cimato TR, Tang J, Xu Y et al (2002) Nerve growth factor-mediated increases in protein methylation occur predominantly at type I arginine methylation sites and involve protein arginine methyltransferase 1. J Neurosci Res 67, 435-442   DOI
33 Hall GF and Yao J (2000) Neuronal morphology, axonal integrity, and axonal regeneration in situ are regulated by cytoskeletal phosphorylation in identified lamprey central neurons. Microsc Res Tech 48, 32-46   DOI
34 Goetz R and Mohammadi M (2013) Exploring mechanisms of FGF signalling through the lens of structural biology. Nat Rev Mol Cell Biol 14, 166-180   DOI
35 Nepal S and Park PH (2014) Regulatory role of autophagy in globular adiponectin-induced apoptosis in cancer cells. Biomol Ther (Seoul) 22, 384-389   DOI
36 Cimato TR, Ettinger MJ, Zhou X and Aletta JM (1997) Nerve growth factor-specific regulation of protein methylation during neuronal differentiation of PC12 cells. J Cell Biol 138, 1089-1103   DOI
37 Hong S, Heo J, Lee S et al (2008) Methyltransferase-inhibition interferes with neuronal differentiation of P19 embryonal carcinoma cells. Biochem Biophys Res Commun 377, 935-940   DOI
38 Pereira JD, Sansom SN, Smith J et al (2010) Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex. Proc Natl Acad Sci U S A 107, 15957-15962   DOI
39 Yoon SY, Kang HB, Ko YE et al (2015) 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) Modulates Th2 Immunity through Attenuation of IL-4 Expression. Immune Netw 15, 100-109   DOI
40 Baek KS, Hong YD, Kim Y et al (2015) Anti-inflammatory activity of AP-SF, a ginsenoside-enriched fraction, from Korean ginseng. J Ginseng Res 39, 155-161   DOI
41 Kim S, Hong JW, Cho WD et al (2014) Characterization of Two Novel mAbs Recognizing Different Epitopes on CD43. Immune Netw 14, 164-170   DOI