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http://dx.doi.org/10.5483/BMBRep.2016.49.7.062

Methyltransferase and demethylase profiling studies during brown adipocyte differentiation  

Son, Min Jeong (Metabolic Regulation Research Center, KRIBB)
Kim, Won Kon (Metabolic Regulation Research Center, KRIBB)
Oh, Kyoung-Jin (Metabolic Regulation Research Center, KRIBB)
Park, Anna (Metabolic Regulation Research Center, KRIBB)
Lee, Da Som (Metabolic Regulation Research Center, KRIBB)
Han, Baek Soo (Metabolic Regulation Research Center, KRIBB)
Lee, Sang Chul (Metabolic Regulation Research Center, KRIBB)
Bae, Kwang-Hee (Metabolic Regulation Research Center, KRIBB)
Publication Information
BMB Reports / v.49, no.7, 2016 , pp. 388-393 More about this Journal
Abstract
Although brown adipose tissue is important with regard to energy balance, the molecular mechanism of brown adipocyte differentiation has not been extensively studied. Specifically, regulation factors at the level of protein modification are largely unknown. In this study, we examine the changes in the expression level of enzymes which are involved in protein lysine methylation during brown adipocyte differentiation. Several enzymes, in this case SUV420H2, PRDM9, MLL3 and JHDM1D, were found to be up-regulated. On the other hand, Set7/9 was significantly down-regulated. In the case of SUV420H2, the expression level increased sharply during brown adipocyte differentiation, whereas the expression of SUV420H2 was marginally enhanced during the white adipocyte differentiation. The knock-down of SUV420H2 caused the suppression of brown adipocyte differentiation, as compared to a scrambled control. These results suggest that SUV420H2, a methyltransferase, is involved in brown adipocyte differentiation, and that the methylation of protein lysine is important in brown adipocyte differentiation.
Keywords
Brown adipocytes; Demethylase; Methyltransferase; Obesity; SUV420H2;
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Times Cited By KSCI : 4  (Citation Analysis)
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