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http://dx.doi.org/10.5483/BMBRep.2016.49.7.048

PEP-1-GSTpi protein enhanced hippocampal neuronal cell survival after oxidative damage  

Sohn, Eun Jeong (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Shin, Min Jea (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Kim, Dae Won (Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangnung-Wonju National University)
Son, Ora (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Jo, Hyo Sang (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Cho, Su Bin (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Park, Jung Hwan (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Lee, Chi Hern (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Yeo, Eun Ji (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Choi, Yeon Joo (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Yu, Yeon Hee (Department of Anatomy, College of Medicine, Soonchunhyang University)
Kim, Duk-Soo (Department of Anatomy, College of Medicine, Soonchunhyang University)
Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine)
Kwon, Oh Shin (School of Life Sciences, College of Natural Sciences, Kyungpook National University)
Cho, Yong-Jun (Department of Neurosurgery, Hallym University Medical Center)
Park, Jinseu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Eum, Won Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Choi, Soo Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Publication Information
BMB Reports / v.49, no.7, 2016 , pp. 382-387 More about this Journal
Abstract
Reactive oxygen species generated under oxidative stress are involved in neuronal diseases, including ischemia. Glutathione S-transferase pi (GSTpi) is a member of the GST family and is known to play important roles in cell survival. We investigated the effect of GSTpi against oxidative stress-induced hippocampal HT-22 cell death, and its effects in an animal model of ischemic injury, using a cell-permeable PEP-1-GSTpi protein. PEP-1-GSTpi was transduced into HT-22 cells and significantly protected against H2O2-treated cell death by reducing the intracellular toxicity and regulating the signal pathways, including MAPK, Akt, Bax, and Bcl-2. PEP-1-GSTpi transduced into the hippocampus in animal brains, and markedly protected against neuronal cell death in an ischemic injury animal model. These results indicate that PEP-1-GSTpi acts as a regulator or an antioxidant to protect against oxidative stress-induced cell death. Our study suggests that PEP-1-GSTpi may have potential as a therapeutic agent for the treatment of ischemia and a variety of oxidative stress-related neuronal diseases.
Keywords
Apoptotic signals; Ischemia; Oxidative stress; PEP-1-GSTpi; Protein therapy;
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