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http://dx.doi.org/10.5483/BMBRep.2016.49.3.219

Loss of phospholipase D2 impairs VEGF-induced angiogenesis  

Lee, Chang Sup (Department of Life Sciences, Pohang University of Science and Technology (POSTECH))
Ghim, Jaewang (Department of Life Sciences, Pohang University of Science and Technology (POSTECH))
Song, Parkyong (Department of Life Sciences, Pohang University of Science and Technology (POSTECH))
Suh, Pann-Ghill (School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST))
Ryu, Sung Ho (Department of Life Sciences, Pohang University of Science and Technology (POSTECH))
Publication Information
BMB Reports / v.49, no.3, 2016 , pp. 191-196 More about this Journal
Abstract
Vascular endothelial growth factor (VEGF) is a key mediator of angiogenesis and critical for normal embryonic development and repair of pathophysiological conditions in adults. Although phospholipase D (PLD) activity has been implicated in angiogenic processes, its role in VEGF signaling during angiogenesis in mammals is unclear. Here, we found that silencing of PLD2 by siRNA blocked VEGF-mediated signaling in immortalized human umbilical vein endothelial cells (iHUVECs). Also, VEGF-induced endothelial cell survival, proliferation, migration, and tube formation were inhibited by PLD2 silencing. Furthermore, while Pld2-knockout mice exhibited normal development, loss of PLD2 inhibited VEGF-mediated ex vivo angiogenesis. These findings suggest that PLD2 functions as a key mediator in the VEGF-mediated angiogenic functions of endothelial cells.
Keywords
Angiogenesis; Aorta ring; Endothelial cells; Phospholipase D; Tube formation; VEGF;
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Times Cited By KSCI : 2  (Citation Analysis)
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