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http://dx.doi.org/10.5483/BMBRep.2016.49.2.202

Epac2 contributes to PACAP-induced astrocytic differentiation through calcium ion influx in neural precursor cells  

Seo, Hyunhyo (Department of Anatomy, Brain Science & Engineering Institute, Kyungpook National University Graduate School of Medicine)
Lee, Kyungmin (Department of Anatomy, Brain Science & Engineering Institute, Kyungpook National University Graduate School of Medicine)
Publication Information
BMB Reports / v.49, no.2, 2016 , pp. 128-133 More about this Journal
Abstract
Astrocytes play a critical role in normal brain functions and maintaining the brain microenvironment, and defects in astrocytogenesis during neurodevelopment could give rise to severe mental illness and psychiatric disorders. During neuro-embryogenesis, astrocytogenesis involves astrocytic differentiation of neural precursor cells (NPCs) induced by signals from ciliary neurotrophic factor (CNTF) or pituitary adenylate cyclase-activating peptide (PACAP). However, in contrast to the CNTF signaling pathway, the exact mechanism underlying astrocytic differentiation induced by PACAP is unknown. In the present study, we aimed to verify a signaling pathway specific to PACAP-induced astrocytogenesis, using exchange protein directly activated by cAMP2 (Epac2)-knockout mice. We found that PACAP could trigger astrocytic differentiation of NPCs via Epac2 activation and an increase in the intracellular calcium concentration via a calcium ion influx. Taken together, we concluded that astrocytogenesis stimulated by PACAP occurs through a novel signaling pathway independent from CNTF-JAK/STAT signaling, that is the well-known pathway of astrocytogenesis.
Keywords
Astrocytogenesis; Calcium ion; Exchange protein directly activated by cAMP2 (Epac2); Neural precursor cells (NPCs); Pituitary adenylate cyclase-activating peptide (PACAP);
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