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http://dx.doi.org/10.5483/BMBRep.2016.49.11.151

Expression and characterization of transmembrane and coiled-coil domain family 3  

Sohn, Wern-Joo (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
Kim, Jae-Young (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
Kim, Dongbum (Center for Medical Science Research, College of Medicine, Hallym University)
Park, Jeong-A (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Lee, Younghee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Kwon, Hyung-Joo (Center for Medical Science Research, College of Medicine, Hallym University)
Publication Information
BMB Reports / v.49, no.11, 2016 , pp. 629-634 More about this Journal
Abstract
Transmembrane and coiled-coil domain family 3 (TMCC3) has been reported to be expressed in the human brain; however, its function is still unknown. Here, we found that expression of TMCC3 is higher in human whole brain, testis and spinal cord compared to other human tissues. TMCC3 was expressed in mouse developing hind brain, lung, kidney and somites, with strongest expression in the mesenchyme of developing tongue. By expression of recombinant TMCC3 and its deletion mutants, we found that TMCC3 proteins self-assemble to oligomerize. Immunostaining and confocal microscopy data revealed that TMCC3 proteins are localized in endoplasmic reticulum through transmembrane domains. Based on immunoprecipitation and mass spectroscopy data, TMCC3 proteins associate with TMCC3 and 14-3-3 proteins. This supports the idea that TMCC3 proteins form oligomers and that 14-3-3 may be involved in the function of TMCC3. Taken together, these results may be useful for better understanding of uncharacterized function of TMCC3.
Keywords
Domains; Localization; Tissue expression; TMCC3; 14-3-3;
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1 Michielse CB, Bhat M, Brady A et al (2006) Refinement of the locus for hereditary congenital facial palsy on chromosome 3q21 in two unrelated families and screening of positional candidate genes. Eur J Hum Genet 14, 1306-1312   DOI
2 Zhang C, Kho YS, Wang Z et al (2014) Transmembrane and coiled-coil domain family 1 is a novel protein of the endoplasmic reticulum. PLoS One 9, e85206   DOI
3 Hirosawa M, Nagase T, Ishikawa K, Kikuno R, Nomura N and Ohara O (1999) Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. DNA Res 6, 329-336   DOI
4 Jorgensen JR, Juliusson B, Henriksen KF et al (2006) Identification of novel genes regulated in the developing human ventral mesencephalon. Exp Neurol 198, 427-437   DOI
5 Gerhard DS, Wagner L, Feingold EA et al (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res 14, 2121-2127   DOI
6 Bennett EJ, Rush J, Gygi SP and Harper JW (2010) Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 143, 951-965   DOI
7 Rose JE, Behm FM, Drgon T, Johnson C and Uhl GR (2010) Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Mol Med 16, 247-253
8 Neupane S, Sohn WJ, Rijal G et al (2014) Developmental regulations of Perp in mice molar morphogenesis. Cell Tissue Res 358, 109-121   DOI
9 Davis OS, Butcher LM, Docherty SJ et al (2010) A three-stage genome-wide association study of general cognitive ability: hunting the small effects. Behav Genet 40, 759-767   DOI
10 Bridges D and Moorhead GB (2005) 14-3-3 proteins: a number of functions for a numbered protein. Sci STKE 2005, re10
11 Na HH, Noh HJ, Cheong HM, Kang Y and Kim KC (2016) SETDB1 mediated FosB expression increases the cell proliferation rate during anticancer drug therapy. BMB Rep 49, 238-243   DOI
12 Cho SH, Park BK, Lee KW, Chang J, Lee Y and Kwon HJ (2015) Effect of respiratory syncytial virus on the growth of hepatocellular carcinoma cell-lines. BMB Rep 48, 565-570   DOI
13 Zuo J, Ma H, Cai H, Wu Y, Jiang W and Yu L (2015) An inhibitory role of NEK6 in $TGF{\beta}/Smad$ signaling pathway. BMB Rep 48, 473-478   DOI
14 Ku SK and Bae JS (2015) Baicalin, baicalein and wogonin inhibits high glucose-induced vascular inflammation in vitro and in vivo. BMB Rep 48, 519-524   DOI
15 Kwon S, Kim D, Rhee JW et al (2010) ASB9 interacts with ubiquitous mitochondrial creatine kinase and inbibits mitochondrial function. BMC Biol 8, 23   DOI