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http://dx.doi.org/10.5483/BMBRep.2015.48.9.117

Gecko proteins induce the apoptosis of bladder cancer 5637 cells by inhibiting Akt and activating the intrinsic caspase cascade  

Kim, Geun-Young (Division of Cardiovascular and Rare Disease, Center for Biomedical Sciences, Korea National Institute of Health)
Park, Soon Yong (Department of Biological Science, Dong-A University)
Jo, Ara (Department of Biological Science, Dong-A University)
Kim, Mira (Department of Biological Science, Dong-A University)
Leem, Sun-Hee (Department of Biological Science, Dong-A University)
Jun, Woo-Jin (Department of Food and Nutrition, Chonnam National University)
Shim, Sang In (Department of Agronomy, Gyeongsang National University)
Lee, Sang Chul (Research Center for Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology)
Chung, Jin Woong (Department of Biological Science, Dong-A University)
Publication Information
BMB Reports / v.48, no.9, 2015 , pp. 531-536 More about this Journal
Abstract
Gecko proteins have long been used as anti-tumor agents in oriental medicine, without any scientific background. Although anti-tumor effects of Gecko proteins on several cancers were recently reported, their effect on bladder cancer has not been investigated. Thus, we explored the anti-tumor effect of Gecko proteins and its cellular mechanisms in human bladder cancer 5637 cells. Gecko proteins significantly reduced the viability of 5637 cells without any cytotoxic effect on normal cells. These proteins increased the Annexin-V staining and the amount of condensed chromatin, demonstrating that the Gecko proteinsinduced cell death was caused by apoptosis. Gecko proteins suppressed Akt activation, and the overexpression of constitutively active form of myristoylated Akt prevented Gecko proteins-induced death of 5637 cells. Furthermore, Gecko proteins activated caspase 9 and caspase 3/7. Taken together, our data demonstrated that Gecko proteins suppressed the Akt pathway and activated the intrinsic caspase pathway, leading to the apoptosis of bladder cancer cells. [BMB Reports 2015; 48(9): 531-536]
Keywords
Akt; Apoptosis; Bladder cancer; Caspase; Gecko proteins;
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