Browse > Article
http://dx.doi.org/10.5483/BMBRep.2015.48.8.215

Acceleration of heat shock-induced collagen breakdown in human dermal fibroblasts with knockdown of NF-E2-related factor 2  

Park, Gunhyuk (Department of Life and Nanopharmaceutical Science, Graduates School and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University)
Oh, Myung Sook (Department of Life and Nanopharmaceutical Science, Graduates School and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University)
Publication Information
BMB Reports / v.48, no.8, 2015 , pp. 467-472 More about this Journal
Abstract
Heat shock increases skin temperature during sun exposure and some evidence indicates that it may be involved in skin aging. The antioxidant response mediated by the transcription factor NF-E2-related factor 2 (Nrf2) is a critically important cellular defense mechanism that serves to limit skin aging. We investigated the effects of heat shock on collagenase expression when the antioxidant defense system was downregulated by knockdown of Nrf2. GSH and collagenases were analyzed, and the expression of inducible Nrf2, HO-1, and NQO1 was measured. HS68 cells were transfected with small interfering RNA against Nrf2. Heat shock induced the downregulation of Nrf2 in both the cytosol and nucleus and reduced the expression of HO-1, GSH, and NQO1. In addition, heat-exposed Nrf2-knockdown cells showed significantly increased levels of collagenase protein and decreased levels of procollagen. Our data suggest that Nrf2 plays an important role in protection against heat shock-induced collagen breakdown in skin. [BMB Reports 2015; 48(8): 467-472]
Keywords
Collagenase; Glutathione; Heat; NF-E2-related factor 2; Si-Nrf2;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Lee EK, Kim JA, Park SJ et al (2013) Low-dose radiation activates Nrf1/2 through reactive species and the Ca2+/ERK1/2 signaling pathway in human skin fibroblast cells. BMB Rep 46, 258-263   DOI
2 Hirota A, Kawachi Y, Yamamoto M, Koga T, Hamada K and Otsuka F (2011) Acceleration of UVB-induced photoageing in Nrf2 gene-deficient mice. Exp Dermatol 20, 664-668   DOI
3 Hirota A, Kawachi Y, Itoh K et al (2005) Ultraviolet A irradiation induces NF-E2-related factor 2 activation in dermal fibroblasts: protective role in UVA-induced apoptosis. J Invest Dermatol 124, 825-832   DOI
4 Chang SW, Lee SI, Bae WJ et al (2009) Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells. J Endod 35, 1222-1228   DOI
5 Sahin K, Orhan C, Akdemir F, Tuzcu M, Iben C and Sahin N (2012) Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins. J Anim Physiol Anim Nutr 96, 66-74   DOI
6 Sahin K, Orhan C, Tuzcu Z, Tuzcu M and Sahin N (2012) Curcumin ameloriates heat stress via inhibition of oxidative stress and modulation of Nrf2/HO-1 pathway in quail. Food Chem Toxicol 50, 4035-4041   DOI
7 Chen X-L and Kunsch C (2004) Induction of cytoprotective genes through Nrf2/antioxidant response element pathway: a new therapeutic approach for the treatment of inflammatory diseases. Curr Pharm Des 10, 879-891   DOI
8 van der Rest M, Garrone R (1991) Collagen family of proteins. FASEB J 5, 2814-2823
9 Chung JH, Seo JY, Choi HR et al (2001) Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol 117, 1218-1224   DOI
10 Yoon BH, Jeon YH, Hwang B et al (2013) Anti-wrinkle effect of bone morphogenetic protein receptor 1a-extracellular domain (BMPR1a-ECD). BMB Rep 46, 465-470   DOI
11 Shin MH, Seo JE, Kim YK et al (2012) Chronic heat treatment causes skin wrinkle formation and oxidative damage in hairless mice. Mech Ageing Dev 133, 92-98   DOI
12 Braun S, Hanselmann C, Gassmann MG et al (2002) Nrf2 transcription factor, a novel target of keratinocyte growth factor action which regulates gene expression and inflammation in the healing skin wound. Mol Cell Biol 22, 5492-5505   DOI
13 Osburn WO and Kensler TW (2008) Nrf2 signaling: an adaptive response pathway for protection against environmental toxic insults. Mutat Res 659, 31-39   DOI
14 Park G, Kim H, Kim Y, Park S, Kim S and Oh M (2012) Coriandrum sativum L. protects human keratinocytes from oxidative stress by regulating oxidative defense systems. Skin Pharmacol Physiol 25, 93-99   DOI
15 Aleksunes LM and Manautou JE (2007) Emerging role of Nrf2 in protecting against hepatic and gastrointestinal disease. Toxicol Pathol 35, 459-473   DOI
16 Zhong JL, Edwards GP, Raval C, Li H and Tyrrell RM (2010) The role of Nrf2 in ultraviolet A mediated heme oxygenase 1 induction in human skin fibroblasts. Photochem Photobiol Sci 9, 18-24   DOI
17 Park G, Jang DS and Oh MS (2012) Juglans mandshurica leaf extract protects skin fibroblasts from damage by regulating the oxidative defense system. Biochem Biophys Res Commun 421, 343-348   DOI
18 Park G, Kim HG, Sim Y, Sung SH and Oh MS (2012) Sauchinone, a Lignan from Saururus chinensis, Protects Human Skin Keratinocytes against Ultraviolet B-Induced Photoaging by Regulating the Oxidative Defense System. Biol Pharm Bull 36, 1134-1139   DOI
19 Park G, Kim H, Hong S-P, Kim S and Oh M (2014) Walnuts (Seeds of Juglandis sinensis L.) Protect Human Epidermal Keratinocytes against UVB-Induced Mitochondria- Mediated Apoptosis through Upregulation of ROS Elimination Pathways. Skin Pharmacol Physiol 27, 132-140   DOI
20 Zastrow L, Groth N, Klein F et al (2008) The missing link-light-induced (280-1,600 nm) free radical formation in human skin. Skin Pharmacol Physiol 22, 31-44
21 Schröder P and Krutmann J (2005) Environmental oxidative stress-Environmental sources of ROS. The handbook of Environmental Chemistry 2, 19-31.
22 Park C-H, Lee MJ, Ahn J et al (2004) Heat shock-induced matrix metalloproteinase (MMP)-1 and MMP-3 are mediated through ERK and JNK activation and via an autocrine interleukin-6 loop. J Invest Dermatol 123, 1012-1019   DOI
23 Schieke SM, Schroeder P and Krutmann J (2003) Cutaneous effects of infrared radiation: from clinical observations to molecular response mechanisms. Photodermatol Photoimmunol Photomed 19, 228-234   DOI
24 Cho S, Lee MJ, Kim MS et al (2008) Infrared plus visible light and heat from natural sunlight participate in the expression of MMPs and type I procollagen as well as infiltration of inflammatory cell in human skin in vivo. J Dermatol Sci 50, 123-133   DOI
25 Shin MH, Moon YJ, Seo J-E, Lee Y, Kim KH and Chung JH (2008) Reactive oxygen species produced by NADPH oxidase, xanthine oxidase, and mitochondrial electron transport system mediate heat shock-induced MMP-1 and MMP-9 expression. Free Radic Biol Med 44, 635-645   DOI
26 Lee YM, Li WH, Kim YK, Kim KH and Chung JH (2008) Heat-induced MMP-1 expression is mediated by TRPV1 through PKCα signaling in HaCaT cells. Exp Dermatol 17, 864-870   DOI