[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5483/BMBRep.2015.48.6.073

Anticancer activity of CopA3 dimer peptide in human gastric cancer cells

Lee, Joon Ha (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, In-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, Sang-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Ahn, Mi-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kang, Dong-Chul (Ilsong Institute of Life Science, Hallym University)
Hwang, Jae Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)

Publication Information

BMB Reports / v.48, no.6, 2015 , pp. 324-329 More about this Journal

Abstract

CopA3 is a homodimeric ${\alpha}$ -helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic. [BMB Reports 2015; 48(6): 324-329]

Keywords

Antimicrobial peptide; Anticancer activity; CopA3; Necrosis; Phosphatidylserine;

Citations & Related Records

Times Cited By KSCI : 3 (Citation Analysis)

Reference
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