Browse > Article
http://dx.doi.org/10.5483/BMBRep.2015.48.5.131

The effect of baicalin in a mouse model of retinopathy of prematurity  

Jo, Hyoung (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Jung, Sang Hoon (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Yim, Hye Bin (Department of Ophthalmology, Incheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea)
Lee, Sung Jin (Department of Ophthalmology, College of Medicine, Soonchunhyang University)
Kang, Kui Dong (Department of Ophthalmology, Incheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea)
Publication Information
BMB Reports / v.48, no.5, 2015 , pp. 271-276 More about this Journal
Abstract
Baicalin is a flavonoid derived from the dried root of Scutellaria baicalensis. In this study, oxygen-induced retinopathy was used to characterize the anti-angiogenic properties of baicalin in mice. Pups were exposed to a hyperbaric oxygen environment to induce retinal angiogenesis and were subjected to intraperitoneal injection of baicalin. Avascular area, neovascular tufts, and neovascular lumens were quantified from digital images. Compared to the vehicle, baicalin clearly reduced the central avascular zone and the number of neovascular tufts and lumens. High-dose baicalin (10 mg/kg) significantly reduced the expression of matrix metalloproteinase-2 (MMP-2), MMP-9, angiotensin II, and vascular endothelial growth factor (VEGF). These results show that baicalin is a powerful antiangiogenic compound that attenuates new vessel formation in the retina after systemic administration, and is a candidate substance for therapeutic inhibition of retinal angiogenesis. [BMB Reports 2015; 48(5): 271-276]
Keywords
Angiogenesis; Angiotensin II; Baicalin; Oxygen-induced retinopathy; VEGF;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Autrata R, Holousová M and Rehůrek J (2002) Cryotherapy and photocoagulation in the treatment of retinopathy of prematurity. Cesk Slov Oftalmol 58, 30-35
2 Chow LC, Wright KW and Sola A (2001) Can changes in clinical practice decrease the incidence of severe retinopathy of prematurity in very low birth weight infants? Pediatrics 111, 339-345   DOI   ScienceOn
3 Shalev B, Farr AK and Repka MX (2001) Randomized comparison of diode laser photocoagulation versus cryotherapy for threshold retinopathy of prematurity: seven-year outcome. Am J Ophthalmol 132, 76-80   DOI   ScienceOn
4 McDonald HR and Schatz H (1985) Visual loss following panretinal photocoagulation for proliferative diabetic retinopathy. Ophthalmology 92, 388-393   DOI
5 Lerner BC, Lakhanpal V and Schocket SS (1984) Transient myopia and accommodative paresis following retinal cryotherapy and panretinal photocoagulation. Am J Ophthalmol 97, 704-708   DOI
6 Liu JJ, Huang TS, Cheng WF and Lu FJ (2003) Baicalein and baicalin are potent inhibitors of angiogenesis: Inhibition of endothelial cell proliferation, migration and differentiation. Int J Cancer 106,559-565   DOI   ScienceOn
7 Miocinovic R, McCabe NP, Keck RW, Jankun J, Hampton JA and Selman SH (2005) In vivo and in vitro effect of baicalein on human prostate cancer cells. Int J Oncol 26, 241-246
8 Zhang K, Lu J, Mori T et al (2010) Baicalin increases VEGF expression and angiogenesis by activating the ERR{alpha}/PGC-1{alpha} pathway. Cardiovasc Res 89, 426-435   DOI
9 Shieh DE, Liu LT and Lin CC (2000) Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res 20, 2861-2865
10 Yang SJ, Jo H, Kim JG and Jung SH (2014) Baicalin Attenuates Laser-Induced Choroidal Neovascularization. Curr Eye Res 39, 745-751   DOI   ScienceOn
11 Deng YF, Aluko RE, Jin Q, Zhang Y and Yuan LJ (2012) Inhibitory activities of baicalin against renin and angiotensin-converting enzyme. Pharm Biol 50, 401-406   DOI   ScienceOn
12 Sarlos S, Rizkalla B, Moravski CJ, Cao Z, Cooper ME and Wilkinson-Berka JL (2003) Retinal angiogenesis is mediated by an interaction between the angiotensin type 2 receptor, VEGF, and angiopoietin. Am J Pathol 163, 879-887   DOI   ScienceOn
13 Huang WH, Lee AR and Yang CH (2006) Antioxidative and anti-inflammatory activities of polyhydroxyflavonoids of Scutellaria baicalensis GEORGI. Biosci Biotechnol Biochem 70, 2371-2380   DOI   ScienceOn
14 Smith LE, Wesolowski E, McLellan A et al (1994) Oxygeninduced retinopathy in the mouse. Invest Ophthalmol Vis Sci 35, 101-111
15 Stahl A, Connor KM, Sapieha P et al (2009) Computeraided quantification of retinal neovascularization. Angiogenesis 12, 297-301   DOI
16 Jo H, Jung SH, Kang J, Yim HB and Kang KD (2014) Sulodexide inhibits retinal neovascularization in a mouse model of oxygen-induced retinopathy. BMB Rep 47, 637-642   DOI   ScienceOn
17 Nanni C, Pettinato C, Ambrosini V et al (2007) Retro-orbital injection is an effective route for radiopharmaceutical administration in mice during small-animal PET studies. Nucl Med Commun 28, 547-553   DOI   ScienceOn
18 Connor KM, Krah NM, Dennison RJ et al (2009) Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis. Nat Protoc 4, 1565-1573   DOI   ScienceOn
19 Lim JH (2014) Zinc finger and BTB domain-containing protein 3 is essential for the growth of cancer cells. BMB Rep 47, 405-410   DOI   ScienceOn