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http://dx.doi.org/10.5483/BMBRep.2015.48.3.105

Resveratrol and clofarabine induces a preferential apoptosis-activating effect on malignant mesothelioma cells by Mcl-1 down-regulation and caspase-3 activation  

Lee, Yoon-Jin (Soonchunhyung Environmental Health Center for Asbestos, College of Medicine, Soonchunhyang University)
Lee, Yong-Jin (Soonchunhyung Environmental Health Center for Asbestos, College of Medicine, Soonchunhyang University)
Lee, Sang-Han (Soonchunhyung Environmental Health Center for Asbestos, College of Medicine, Soonchunhyang University)
Publication Information
BMB Reports / v.48, no.3, 2015 , pp. 166-171 More about this Journal
Abstract
We previously demonstrated that resveratrol and clofarabine elicited a marked cytotoxicity on malignant mesothelioma (MM) MSTO-211H cells but not on the corresponding normal mesothelial MeT-5A cells. Little is known of the possible molecules that could be used to predict preferential chemosensitivity on MSTO-211H cells. Resveratrol and clofarabine induced downregulation of Mcl-1 protein level in MSTO-211H cells. Treatment of cells with cycloheximide in the presence of proteasome inhibitor MG132 suggested that Mcl-1 protein levels were regulated at the post-translational step. The siRNA-based knockdown of Mcl-1 in MSTO-211H cells triggered more growth-inhibiting and apoptosis-inducing effects with the resultant cleavages of procaspase-3 and its substrate PARP, increased caspase-3/7 activity, and increased percentage of apoptotic propensities. However, the majority of the observed changes were not shown in MeT-5A cells. Collectively, these studies indicate that the preferential activation of caspase cascade in malignant cells might have important applications as a therapeutic target for MM.
Keywords
Chemosensitivity; Clofarabine; MeT-5A; MSTO-211H; Resveratrol;
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1 Moulding DA, Giles RV, Spiller DG, White MR, Tidd DM and Edwards SW (2000) Apoptosis is rapidly triggered by antisense depletion of MCL-1 in differentiating U937 cells. Blood 96, 1756-1763
2 Thallinger C, Wolschek MF, Wacheck V et al (2003) Mcl-1 antisense therapy chemosensitizes human melanoma in a SCID mouse xenotransplantation model. J Invest Dermatol 120, 1081-1096   DOI   ScienceOn
3 Nencioni A, Hua F, Dillon CP et al (2005) Evidence for a protective role of Mcl-1 in proteasome inhibitor-induced apoptosis. Blood 105, 3255-3262   DOI   ScienceOn
4 Han J, Goldstein LA and Gastman BR (2006) Interrelated roles for Mcl-1 and BIM in regulation of TRAIL-mediated mitochondrial apoptosis. J Biol Chem 281, 10153-10163   DOI   ScienceOn
5 Maurer U, Charvet C, Wagman AS, Dejardin E and Green DR (2006) Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of Mcl-1. Mol. Cell 21, 749-760   DOI   ScienceOn
6 Pozo-Guisado E, Merino JM, Mulero-Navarro S et al (2005) Resveratrol-induced apoptosis in MCF-7 human breast cancer cells involves a caspase-independent mechanism with downregulation of Bcl-2 and NF-kappaB. Int J Cancer 115, 74-84   DOI   ScienceOn
7 Tolomeo M, Grimaudo S, Di Cristina A et al (2005) Pterostilbene and 3'-hydroxypterostilbene are effective apoptosis-inducing agents in MDR and BCR-ABL-expressing leukemia cells. Int J Biochem Cell Biol 37, 1709-1726   DOI   ScienceOn
8 Augustine CK, Toshimitsu H, Jung SH et al (2010) Sorafenib, a multikinase inhibitor, enhances the response of melanoma to regional chemotherapy. Mol Cancer Ther 9, 2090-2101   DOI
9 Lee YJ, Im JH, Lee DM et al (2012) Synergistic inhibition of mesothelioma cell growth by the combination of clofarabine and resveratrol involves Nrf2 downregulation. BMB Rep 45, 647-652   DOI   ScienceOn
10 Simonin K, Brotin E, Dufort S et al (2009) Mcl-1 is an important determinant of the apoptotic response to the BH3-mimetic molecule HA14-1 in cisplatin-resistant ovarian carcinoma cells. Mol Cancer Ther 8, 3162-3170   DOI   ScienceOn
11 Kitada S, Anderson J and Akar S (1998) Expression of apoptosis-regulating proteins in chronic lymphocytic leukemia: correlations with in vitro and in vivo chemoresponses. Blood 91, 3379-3389
12 Lee YJ, Lee YJ, Im JH et al (2013) Synergistic anti-cancer effects of resveratrol and chemotherapeutic agent clofarabine against human malignant mesothelioma MSTO-211H cells. Food Chem Toxicol 52, 61-68   DOI   ScienceOn
13 Shroff EH, Snyder C, Chandel NS (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal 9, 1405-1409   DOI   ScienceOn
14 O'Kane SL, Pound RJ, Campbell A, Chaudhuri N, Lind MJ and Cawkwell L (2006) Expression of bcl-2 family members in malignant pleural mesothelioma. Acta Oncol 45, 449-453   DOI   ScienceOn
15 Craig RW (2002) MCL1 provides a window on the role of the BCL2 family in cell proliferation, differentiation and tumorigenesis. Leukemia 16, 444-454   DOI   ScienceOn
16 Nijhawan D, Fang M, Traer E et al (2003) Elimination of Mcl-1 is required for the initiation of apoptosis following ultraviolet irradiation. Genes Dev 17, 1475-1486   DOI   ScienceOn
17 Montanaro F, Rosato R, Gangemi M et al (2009) Survival of pleural malignant mesothelioma in Italy: a population-based study. Int J Cancer 124, 201-207   DOI   ScienceOn
18 Barbone D, Cheung P, Battula S et al (2012) Vorinostat eliminates multicellular resistance of mesothelioma 3D spheroids via restoration of Noxa expression. PLoS One 7, e52753   DOI
19 Grimm D, Wehland M, Pietsch J, Infanger M and Bauer J (2011) Drugs interfering with apoptosis in breast cancer. Curr Pharm Des 17, 272-283   DOI   ScienceOn
20 Soini Y, Kinnula V, Kaarteenaho-Wiik R, Kurttila E, Linnainmaa K and Pääkkö P (1999) Apoptosis and expression of apoptosis regulating proteins bcl-2, mcl-1, bcl-X, and bax in malignant mesothelioma. Clin Cancer Res 5, 3508-3515
21 Shimazu T, Degenhardt K, Nur-E-Kamal A et al (2007) NBK/BIK antagonizes MCL-1 and BCL-XL and activates BAK-mediated apoptosis in response to protein synthesis inhibition. Genes Dev 21, 929-941   DOI   ScienceOn
22 Kaufmann SH, Karp JE, Svingen PA et al (1998) Elevated expression of the apoptotic regulator Mcl-1 at the time of leukemic relapse. Blood 91, 991-1000