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http://dx.doi.org/10.5483/BMBRep.2015.48.1.022

Proteomic analysis of differentially expressed skin proteins in iRhom2Uncv mice  

Liu, Bing (Institute of JingFeng Medical Laboratory Animal)
Xu, Yuan (Institute of JingFeng Medical Laboratory Animal)
Li, Wen-Long (Institute of JingFeng Medical Laboratory Animal)
Zeng, Lin (Institute of JingFeng Medical Laboratory Animal)
Publication Information
BMB Reports / v.48, no.1, 2015 , pp. 19-24 More about this Journal
Abstract
A mouse homozygous for the spontaneous mutation uncovered (Uncv) has a hairless phenotype. A 309-bp non-frameshift deletion mutation in the N-terminal cytoplasmic domain of iRhom2 was identified in Uncv mice ($iRhom2^{Uncv}$) using target region sequencing. The detailed molecular basis for how the iRhom2 mutation causes the hairless phenotype observed in the homozygous $iRhom2^{Uncv}$ mouse remains unknown. To identify differentially expressed proteins in the skin of wild-type and homozygous $iRhom2^{Uncv}$ littermates at postnatal day 5, proteomic approaches, including two-dimensional gel electrophoresis and mass spectrometry were used. Twelve proteins were differentially expressed in the skin in a comparison between wild-type and homozygous $iRhom2^{Uncv}$ mice. A selection of the proteomic results were tested and verified using qRT-PCR, western blot and immunohistochemistry. These data indicate that differentially expressed proteins, especially KRT73, MEMO1 and Coro-1, might participate in the mechanism by which iRhom2 regulates the development of murine skin.
Keywords
Hair follicle; iRhom2; Proteomic; Skin; Uncv;
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