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http://dx.doi.org/10.5483/BMBRep.2015.48.10.003

Mangiferin inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 expression and cellular invasion by suppressing nuclear factor-κB activity  

Dilshara, Matharage Gayani (Department of Marine Life Sciences, Jeju National University)
Kang, Chang-Hee (Department of Marine Life Sciences, Jeju National University)
Choi, Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
Kim, Gi-Young (Department of Marine Life Sciences, Jeju National University)
Publication Information
BMB Reports / v.48, no.10, 2015 , pp. 559-564 More about this Journal
Abstract
We investigated the effects of mangiferin on the expression and activity of metalloproteinase (MMP)-9 and the invasion of tumor necrosis factor (TNF)-$\alpha$-stimulated human LNCaP prostate carcinoma cells. Reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis showed that mangiferin significantly reversed TNF-$\alpha$-induced mRNA and protein expression of MMP-9 expression. Zymography data confirmed that stimulation of cells with TNF-$\alpha$ significantly increased MMP-9 activity. However, mangiferin substantially reduced the TNF-$\alpha$-induced activity of MMP-9. Additionally, a matrigel invasion assay showed that mangiferin significantly reduced TNF-$\alpha$-induced invasion of LNCaP cells. Compared to untreated controls, TNF-$\alpha$-stimulated LNCaP cells showed a significant increase in nuclear factor-${\kappa}B$ (NF-${\kappa}B$) luciferase activity. However, mangiferin treatment markedly decreased TNF-$\alpha$-induced NF-${\kappa}B$ luciferase activity. Furthermore, mangiferin suppressed nuclear translocation of the NF-${\kappa}B$ subunits p65 and p50. Collectively, our results indicate that mangiferin is a potential anti-invasive agent that acts by suppressing NF-${\kappa}B$-mediated MMP-9 expression.
Keywords
Invasion; Mangiferin; Matrix metalloproteinase-9; Nuclear factor kappa B;
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