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http://dx.doi.org/10.5483/BMBRep.2014.47.9.149

Oleanolic acid regulates NF-κB signaling by suppressing MafK expression in RAW 264.7 cells  

Hwang, Yu-Jin (Department of Agrofood Resources, National Academy of Agricultural Science, RDA)
Song, Jaewhan (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University)
Kim, Haeng-Ran (Department of Agrofood Resources, National Academy of Agricultural Science, RDA)
Hwang, Kyung-A (Department of Agrofood Resources, National Academy of Agricultural Science, RDA)
Publication Information
BMB Reports / v.47, no.9, 2014 , pp. 524-529 More about this Journal
Abstract
Oxidative stress and inflammation are common to many pathological conditions. Defense mechanisms protect cells from oxidative stress, but can become over-activated following injury and inflammation. NF-${\kappa}B$ and Nrf2 transcription factors regulate proinflammatory and antioxidant gene expression, respectively. Studies have shown that many natural dietary compounds regulate NF-${\kappa}B$ and Nrf2, preventing inflammation and oxidative stress. Here, we report major compounds of Prunella vulgaris var. lilacina such as rosmarinic acid, oleanolic acid, ursolic acid and caffeic acid as a potential therapeutic for oxidative stress and inflammation. The major compounds exhibited high anti-inflammatory activity, inhibiting NO, PGE2 production, NF-${\kappa}B$ expression and activating Nrf2 expression. In addition, we examined the effect of major compounds on MafK expression. Among the compounds, oleanolic acid significantly decreased MafK expression and MafK-mediated p65 acetylation. These findings suggest that oleanolic acid as NF-${\kappa}B$ inhibitors can potentially be used in therapeutic applications for the treatment of oxidative stress-induced diseases.
Keywords
MafK; NF-${\kappa}B$; Nrf2; Oleanolic acid; p65 acetylation;
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