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http://dx.doi.org/10.5483/BMBRep.2014.47.9.001

Mycobacterium abscessus MAB2560 induces maturation of dendritic cells via Toll-like receptor 4 and drives Th1 immune response  

Lee, Su Jung (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, School of Medicine, Konkuk University)
Shin, Sung Jae (Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine)
Lee, Seung Jun (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, School of Medicine, Konkuk University)
Lee, Moon Hee (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, School of Medicine, Konkuk University)
Kang, Tae Heung (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, School of Medicine, Konkuk University)
Noh, Kyung Tae (Department of Infectious Diseases Research, Armed Forces Medical Research Institute)
Shin, Yong Kyoo (Department of Pharmacology, College of Medicine, Chung-Ang University)
Kim, Han Wool (Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University)
Yun, Cheol-Heui (Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University)
Jung, In Duk (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, School of Medicine, Konkuk University)
Park, Yeong-Min (Department of Microbiology and Immunology, School of Medicine, Pusan National University)
Publication Information
BMB Reports / v.47, no.9, 2014 , pp. 512-517 More about this Journal
Abstract
In this study, we showed that Mycobacterium abscessus MAB2560 induces the maturation of dendritic cells (DCs), which are representative antigen-presenting cells (APCs). M. abscessus MAB2560 stimulate the production of pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor (TNF)-${\alpha}$, IL-$1{\beta}$, and IL-12p70] and reduce the endocytic capacity and maturation of DCs. Using $TLR4^{-/-}$ DCs, we found that MAB2560 mediated DC maturation via Toll-like receptor 4 (TLR4). MAB2560 also activated the MAPK signaling pathway, which was essential for DC maturation. Furthermore, MAB2560-treated DCs induced the transformation of $na\ddot{i}ve$ T cells to polarized $CD4^+$ and $CD8^+$ T cells, which would be crucial for Th1 polarization of the immune response. Taken together, our results indicate that MAB2560 could potentially regulate the host immune response to M. abscessus and may have critical implications for the manipulation of DC functions for developing DC-based immunotherapy.
Keywords
Dendritic cells; MAB2560; MAPKs; Mycobacterium abscessus; Th1 polarization;
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