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http://dx.doi.org/10.5483/BMBRep.2014.47.4.137

Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP+-dependent isocitrate dehydrogenase in HaCaT cells  

Lee, Su Jeong (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
Park, Jeen-Woo (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
Publication Information
BMB Reports / v.47, no.4, 2014 , pp. 209-214 More about this Journal
Abstract
Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic $NADP^+$-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, by supplying NADPH for antioxidant systems. In this study, we demonstrated that knockdown of IDPc expression by RNA interference enhances UVB-induced apoptosis of immortalized human HaCaT keratinocytes. This effect manifested as DNA fragmentation, changes in cellular redox status, mitochondrial dysfunction, and modulation of apoptotic marker expression. Based on our findings, we suggest that attenuation of IDPc expression may protect skin from UVB-mediated damage, by inducing the apoptosis of UV-damaged cells.
Keywords
Antioxidant enzyme; Apoptosis; Redox status; siRNA; UVB;
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