Browse > Article
http://dx.doi.org/10.5483/BMBRep.2014.47.2.088

Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway  

Lee, Hyeong-Seon (Department of Smart Foods and Drugs, Inje University)
Lee, Gyeong-Seon (Department of Smart Foods and Drugs, Inje University)
Kim, Seon-Hee (Department of Smart Foods and Drugs, Inje University)
Kim, Hyun-Kyung (Department of Biomedical Laboratory Science, College of Natural Science, Gimcheon University)
Suk, Dong-Hee (Department of Smart Foods and Drugs, Inje University)
Lee, Dong-Seok (Department of Smart Foods and Drugs, Inje University)
Publication Information
BMB Reports / v.47, no.2, 2014 , pp. 98-103 More about this Journal
Abstract
Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway.
Keywords
Anti-oxidizing effect; HO-1; MAPK; Nrf2/Keap1; Orostachys japonicus;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Fan, W., Tang, Z., Chen, D., Moughon, D., Ding, X., Chen, S., Zhu, M. and Zhong, Q. (2010) Keap1 facilitates p62- mediated ubiquitin aggregate clearance via autophage. Autophagy 6, 614-621.   DOI   ScienceOn
2 Park, S. Y., Park, D. J., Kim, Y. H., Kim, Y. H., Kim, S. G., Shon, K. J., Choi, Y. W. and Lee, S. J. (2011) Upregulation of heme oxygenase-1 via PI3K/Akt and Nrf2 signaling pathways mediates the anti-inflammatory activity of Schisandrin in Porphyromonas gingivalis LPS-stimulated macrophages. Immunol. Lett. 139, 93-101.   DOI   ScienceOn
3 Lee, J. M. and Johnson, J. A. (2004) An Important Role of Nrf2-ARE Pathway in the Cellular Defense Mechanism. J. Biochem. Mol. Biol. 37, 139-143.   DOI   ScienceOn
4 Stepkowski, T. M. and Kruszewski, M. K. (2011) Molecular cross-talk between the NRF2/KEAP1 signaling pathway, autophagy, and apoptosis. Free Radic. Biol. Med. 50, 1186-1195.   DOI   ScienceOn
5 Manzoor, Z. and Koh, Y. S. (2012) Mitogen-activated Protein Kinases in Inflammation. J. Bacteriol. Virol. 42, 189-195.   DOI   ScienceOn
6 Kim, I. D. and Ha, B. J. (2009) Paeoniflorin protects RAW 264.7 macrophages from LPS-induced cytotoxicity and genotoxicity. Toxicol. In Vitro. 23, 1014-1019.   DOI   ScienceOn
7 Chow, J. M., Shen, S. C., Huan, S. K., Lin, H. Y. and Chen, Y. C. (2005) Quercetin, but not rutin and quercitrin, prevention of H2O2-induced apoptosis via anti-oxidant activity and heme oxygenase 1 gene expression in macrophages. Biochem. Pharmacol. 69, 1839-1851.   DOI   ScienceOn
8 Kim, J. Y., Cha, Y. N. and Surh, Y. J. (2010) Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. Mutat. Res. 690, 12-23.   DOI   ScienceOn
9 Kim, C. K., Jang, J. S., Cho, M. R., Agarawal, S. R. and Cha, Y. N. (2010) Taurine chloramine induces heme oxygenase-1 expression via Nrf2 activation in murine macrophages. Int. Immunopharmacology 10, 440-446.   DOI   ScienceOn
10 Lee, D. S., Jeong, G. S., Li, B., Park, H. and Kim, Y. C. (2010) Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. Int. Immunopharmacology 10, 850-858.   DOI   ScienceOn
11 Park, S. Y., Park, D. J., Kim, Y. H., Kim, Y. H., Choi, Y. W. and Lee, S. J. (2011) Schisandra chinensis $\alpha$-iso-cubebenol induces heme oxygenase-1 expression through PI3K/ Akt and Nrf2 signaling and has anti-inflammatory activity in Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages. Int. Immunopharmacology 11, 1907-1915.   DOI   ScienceOn
12 Park, J. C., Jan, W. D., Park, J. R., Choi, S. H. and Choi, J. W. (2005) Changes in hepatic drug metabolizing enzymes and lipid peroxidation by methanol extract and major compound of Orostachys japonicus. J. Ethnopharmacol. 102, 313-318.   DOI   ScienceOn
13 Khlifi, D., Sghaier, R. M., Amouri, S., Laouini, D., Hamdi, M. and Bouajila, J. (2013) Composition and anti-oxidant, anti- cancer and anti-inflammatory activities of Artemisia herba- alba, Ruta chalpensis L. and Peganum harmala L. Food Chem. Toxicol. 55, 202-208.   DOI   ScienceOn
14 Chung, H. T., Choi, B. M., Kwon, Y. G. and Kim, Y. M. (2008) Interaction Relations between Nitric Oxide (NO) and Carbon Monoxide (CO): Heme Oxygenase-1/CO pathway is a key modulator in NO-mediated Antiapoptosis and Anti-inflammation. Methods Enzymol. 441, 329-338.   DOI   ScienceOn
15 Ryu, D. S., Baek, G. O., Kim, E. Y., Kim, K. H. and Lee, D. S. (2011) Effects of polysaccharides derived from Orostachys japonicus on induction of cell cycle arrest and apoptotic cell death in human colon cancer cells. BMB Rep. 43, 750-755.   과학기술학회마을   DOI   ScienceOn
16 Jeong, J. H., Ryu, D. S., Suk, D. H. and Lee, D. S. (2011) Anti-inflammatory effects of ethanol extract from Orostachys japonicus on modulation of signal pathways in LPSstimulated RAW 264.7 cells. BMB Rep. 44, 399-404.   DOI   ScienceOn
17 Lee, H. S., Ryu, D. S., Lee, G. S. and Lee, D. S. (2012) Antiinflammatory effects of dichloromethane fraction from Orostachys japonicus in RAW 264.7 cells: Suppression of NF-${\kappa}B$ activation and MAPK signaling. J. Ethnopharmacology 140, 271-276.   DOI   ScienceOn
18 Lee, H. S., Bilehal, D., Lee, G. S., Ryu, D. S., Kim, H. K., Suk, D. H. and Lee, D. S. (2013) Anti-inflammatory effect of the hexane fraction from Orostachys japonicus in RAW 264.7 cells by suppression of NF-${\kappa}B$ and PI3K-Akt signaling. J. Funct. Foods 5, 1217-1225.   DOI   ScienceOn
19 Ma, Y. F., Jung, J. Y., Jung, Y. J., Choi, J. H., Jeong, W. S., Song, Y. S., Kang, J. S., Bi, K. and Kim, M. J. (2009) Anti-inflammatory Activity of Coumarins Isolated from Angelica gigas Nakai on LPS-stimulated RAW 264.7 Cells. J. Food Sci. Nut. 14, 179-187.   DOI   ScienceOn
20 Kim, A. N., Jeon, W. K., Lee, J. J. and Kim, B. C. (2010) Up-regulation of heme oxygenase-1 expression through CaMKII-ERK1/2-Nrf2 signaling mediates the anti-inflammatory effect of bisdemethoxycurcumin in LPS-stimulated macrophages. Free Radic. Biol. Med. 49, 323-331.   DOI   ScienceOn
21 Song, J. D., Kim, K. M., Kim, J. M., Yoo, Y. H. and Park, Y. C. (2011) Involvement of Peroxynitrite in NO Doner- Induced HO-1 Expression in Rat Articular Chondrocytes. J. Life Sci.21, 486-493.   과학기술학회마을   DOI   ScienceOn
22 Kim B. C., Choi, J. W., Hong, H. Y., Lee, S. A., Hong, S., Park, E. H., Kim, S. J. and Lim C. J. (2006) Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW 264.7 macrophages. J. Ethnopharmacol. 106, 364-371.   DOI   ScienceOn
23 Copple, I. M., Goldring, C. E., Kitteringham, N. R. and Park, B. K. (2008) The Nrf2-Keap1 defence pathway: Role in protection against drug-induced toxicity. Toxicology 246, 24-33.   DOI   ScienceOn
24 Cho, J. E., Park, S., Lee, H., Cho, S. N. and Kim, Y. S. (2013) Mycobacterium tuberculosis-induced expression of granulocyte- macrophage colony stimulating factor is mediated by PI3-K/MEK1/p38 MAPK signaling pathway. BMB Rep. 46, 213-218.   과학기술학회마을   DOI   ScienceOn