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http://dx.doi.org/10.5483/BMBRep.2014.47.2.117

Sequence-specific interaction between ABD-B homeodomain and castor gene in Drosophila  

Kim, Keon-Hee (Department of Life Sciences, College of Sciences, Yeungnam University)
Yoo, Siuk (Department of Life Sciences, College of Sciences, Yeungnam University)
Publication Information
BMB Reports / v.47, no.2, 2014 , pp. 92-97 More about this Journal
Abstract
We have examined the effect of bithorax complex genes on the expression of castor gene. During the embryonic stages 12-15, both Ultrabithorax and abdominal-A regulated the castor gene expression negatively, whereas Abdominal-B showed a positive correlation with the castor gene expression according to real-time PCR. To investigate whether ABD-B protein directly interacts with the castor gene, electrophoretic mobility shift assays were performed using the recombinant ABD-B homeodomain and oligonucleotides, which are located within the region 10 kb upstream of the castor gene. The results show that ABD-B protein directly binds to the castor gene specifically. ABD-B binds more strongly to oligonucleotides containing two 5'-TTAT-3' canonical core motifs than the probe containing the 5'-TTAC-3' motif. In addition, the sequences flanking the core motif are also involved in the protein-DNA interaction. The results demonstrate the importance of HD for direct binding to target sequences to regulate the expression level of the target genes.
Keywords
Abdominal-B; Castor; EMSA; Homeodomain; Homeotic gene;
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