[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5483/BMBRep.2014.47.1.079

miR-29a suppresses growth and invasion of gastric cancer cells in vitro by targeting VEGF-A

Chen, Ling (Department of Gastroenterology, the 324th Hospital of PLA)
Xiao, Hong (Department of Gastroenterology, the 324th Hospital of PLA)
Wang, Zong-Hua (School of Nursing, Third Military Medical University)
Huang, Yi (Department of Gastroenterology, the 324th Hospital of PLA)
Liu, Zi-Peng (Department of Gastroenterology, the 324th Hospital of PLA)
Ren, Hui (School of Nursing, Third Military Medical University)
Song, Hang (Department of Gastroenterology, the 324th Hospital of PLA)

Publication Information

BMB Reports / v.47, no.1, 2014 , pp. 39-44 More about this Journal

Abstract

Increasing data shows miR-29a is a key regulator of oncogenic processes. It is significantly down-regulated in some kind of human tumors and possibly functionally linked to cellular proliferation, survival and migration. However, the mechanism remains unclear. In this study, we report miR-29a is significantly under-expressed in gastric cancer compared to the healthy donor. The microvessel density is negatively related to miR-29a expression in gastric cancer tissues. The ectopic expression of miR-29a significantly inhibits proliferation and invasion of gastric cancer cells. Furthermore, western blot combined with the luciferase reporter assays demonstrate that vascular endothelial growth factor A (VEGF-A) is direct target of miR-29a. This is the first time miR-29a was found to suppress the tumor microvessel density in gastric cancer by targeting VEGF-A. Taken together, these results suggest that miR-29a is a tumor suppressor in gastric cancer. Restoration of miR-29a in gastric cancer may be a promising therapeutic approach.

Keywords

Gastric cancer; Hsa-miR-29a; MicroRNAs; Post-transcriptional regulation; VEGF-A;

Citations & Related Records

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