Browse > Article
http://dx.doi.org/10.5483/BMBRep.2014.47.10.252

Characterization of the cellular localization of C4orf34 as a novel endoplasmic reticulum resident protein  

Jun, Mi-Hee (Department of Biotechnology, College of Life Science and Nano Technology, Hannam University)
Jun, Young-Wu (Department of Ecological Science, College of Ecology and Environment, Kyungpook National University)
Kim, Kun-Hyung (Department of Ecological Science, College of Ecology and Environment, Kyungpook National University)
Lee, Jin-A (Department of Biotechnology, College of Life Science and Nano Technology, Hannam University)
Jang, Deok-Jin (Department of Ecological Science, College of Ecology and Environment, Kyungpook National University)
Publication Information
BMB Reports / v.47, no.10, 2014 , pp. 563-568 More about this Journal
Abstract
Human genome projects have enabled whole genome mapping and improved our understanding of the genes in humans. However, many unknown genes remain to be functionally characterized. In this study, we characterized human chromosome 4 open reading frame 34 gene (hC4orf34). hC4orf34 was highly conserved from invertebrate to mammalian cells and ubiquitously expressed in the organs of mice, including the heart and brain. Interestingly, hC4orf34 is a novel ER-resident, type I transmembrane protein. Mutant analysis showed that the transmembrane domain (TMD) of hC4orf34 was involved in ER retention. Overall, our results indicate that hC4orf34 is an ER-resident type I transmembrane protein, and might play a role in ER functions including $Ca^{2+}$ homeostasis and ER stress.
Keywords
C4orf32; C4orf34; C4orf52; Endoplasmic reticulum; Type I transmembrane protein;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Morris, J. A., Dorner, A. J., Edwards, C. A., Hendershot, L. M. and Kaufman, R. J. (1997) Immunoglobulin binding protein (BiP) function is required to protect cells from endoplasmic reticulum stress but is not required for the secretion of selective proteins. J. Biol. Chem. 272, 4327-4334.   DOI   ScienceOn
2 Chen, L. C., Liu, M. Y., Hsiao, Y. C., Choong, W. K., Wu, H. Y., Hsu, W. L., Liao, P. C., Sung, T. Y., Tsai, S. F., Yu, J. S. and Chen, Y. J. (2013) Decoding the disease-associated proteins encoded in the human chromosome 4. J. Proteome Res. 12, 33-44.   DOI   ScienceOn
3 Goh, K. I., Cusick, M. E., Valle, D., Childs, B., Vidal, M. and Barabasi, A. L. (2007) The human disease network. Proc. Natl. Acad. Sci. U.S.A. 104, 8685-8690.   DOI   ScienceOn
4 Sachs, J. N. and Engelman, D. M. (2006) Introduction to the membrane protein reviews: the interplay of structure, dynamics, and environment in membrane protein function. Annu. Rev. Biochem. 75, 707-712.   DOI   ScienceOn
5 Ott, C. M. and Lingappa, V. R. (2002) Integral membrane protein biosynthesis: why topology is hard to predict. J. Cell Sci. 115, 2003-2009.
6 Chou, K. C. and Elrod, D. W. (1999) Prediction of membrane protein types and subcellular locations. Proteins 34, 137-153.   DOI
7 Michelsen, K., Yuan, H. and Schwappach, B. (2005) Hide and run. Arginine-based endoplasmic-reticulum-sorting motifs in the assembly of heteromultimeric membrane proteins. EMBO Rep. 6, 717-722.   DOI   ScienceOn
8 Rayner, J. C. and Pelham, H. R. (1997) Transmembrane domain-dependent sorting of proteins to the ER and plasma membrane in yeast. EMBO J. 16, 1832-1841.   DOI   ScienceOn
9 Honsho, M., Mitoma, J. Y. and Ito, A. (1998) Retention of cytochrome b5 in the endoplasmic reticulum is transmembrane and luminal domain-dependent. J. Biol. Chem. 273, 20860-20866.   DOI   ScienceOn
10 Krogh, A., Larsson, B., von Heijne, G. and Sonnhammer, E. L. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305, 567-580.   DOI   ScienceOn
11 Bernsel, A., Viklund, H., Falk, J., Lindahl, E., von Heijne, G. and Elofsson, A. (2008) Prediction of membrane- protein topology from first principles. Proc. Natl. Acad. Sci. U.S.A. 105, 7177-7181.   DOI   ScienceOn
12 Voeltz, G. K., Prinz, W. A., Shibata, Y., Rist, J. M. and Rapoport, T. A. (2006) A class of membrane proteins shaping the tubular endoplasmic reticulum. Cell 124, 573-586.   DOI   ScienceOn
13 Hammond, G. R., Fischer, M. J., Anderson, K. E., Holdich, J., Koteci, A., Balla, T. and Irvine, R. F. (2012) PI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity. Science 337, 727-730.   DOI   ScienceOn
14 Ri, M., Tashiro, E., Oikawa, D., Shinjo, S., Tokuda, M., Yokouchi, Y., Narita, T., Masaki, A., Ito, A., Ding, J., Kusumoto, S., Ishida, T., Komatsu, H., Shiotsu, Y., Ueda, R., Iwawaki, T., Imoto, M. and Iida, S. (2012) Identification of Toyocamycin, an agent cytotoxic for multiple myeloma cells, as a potent inhibitor of ER stress-induced XBP1 mRNA splicing. Blood Cancer J. 2, e79.   DOI   ScienceOn
15 Inoue, T., Heo, W. D., Grimley, J. S., Wandless, T. J. and Meyer, T. (2005) An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways. Nat. Methods 2, 415-418.   DOI   ScienceOn
16 Jang, D. J., Park, S. W., Lee, J. A., Lee, C., Chae, Y. S., Park, H., Kim, M. J., Choi, S. L., Lee, N., Kim, H. and Kaang, B. K. (2010) N termini of apPDE4 isoforms are responsible for targeting the isoforms to different cellular membranes. Learn. Mem. 17, 469-479.   DOI   ScienceOn
17 Jang, D. J., Ban, B. and Lee, J. A. (2011) Characterization of novel calmodulin binding domains within IQ motifs of IQGAP1. Mol. Cells 32, 511-518.   DOI
18 Zarrinpar, A., Bhattacharyya, R. P. and Lim, W. A. (2003) The structure and function of proline recognition domains. Sci STKE 2003, RE8.
19 Voeltz, G. K., Rolls, M. M. and Rapoport, T. A. (2002) Structural organization of the endoplasmic reticulum. EMBO Rep. 3, 944-950.   DOI   ScienceOn
20 Bretscher, M. S. and Munro, S. (1993) Cholesterol and the Golgi apparatus. Science 261, 1280-1281.   DOI
21 Weerapana, E. and Imperiali, B. (2006) Asparagine-linked protein glycosylation: from eukaryotic to prokaryotic systems. Glycobiology 16, 91R-101R.   DOI   ScienceOn
22 Cannon, K. S., Hebert, D. N. and Helenius, A. (1996) Glycan-dependent and -independent association of vesicular stomatitis virus G protein with calnexin. J. Biol. Chem. 271, 14280-14284.   DOI   ScienceOn
23 Suh, B. C., Inoue, T., Meyer, T. and Hille, B. (2006) Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channels. Science 314, 1454-1457.   DOI   ScienceOn
24 Horejsi, V., Zhang, W. and Schraven, B. (2004) Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat. Rev. Immunol. 4, 603-616.   DOI   ScienceOn
25 Hendershot, L. M. (2004) The ER function BiP is a master regulator of ER function. Mt. Sinai. J. Med. 71, 289-297.
26 Neutzner, A., Neutzner, M., Benischke, A. S., Ryu, S. W., Frank, S., Youle, R. J. and Karbowski, M. (2011) A systematic search for endoplasmic reticulum (ER) membrane- associated RING finger proteins identifies Nixin/ZNRF4 as a regulator of calnexin stability and ER homeostasis. J. Biol. Chem. 286, 8633-8643.   DOI   ScienceOn