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http://dx.doi.org/10.5483/BMBRep.2014.47.10.216

Type 3 muscarinic acetylcholine receptor stimulation is a determinant of endothelial barrier function and adherens junctions integrity: role of protein-tyrosine phosphatase 1B  

Jiao, Zhou-Yang (Department of Cardiovascular Surgery, Xiehe Hospital, Huazhong University of Science and Technology)
Wu, Jing (Department of Pediatrics, First Affiliated Hospital of Zhengzhou University)
Liu, Chao (Department of Cardiovascular Surgery, First Affiliated Hospital of Zhengzhou University)
Wen, Bing (Department of Cardiovascular Surgery, First Affiliated Hospital of Zhengzhou University)
Zhao, Wen-Zeng (Department of Cardiovascular Surgery, First Affiliated Hospital of Zhengzhou University)
Du, Xin-Ling (Department of Cardiovascular Surgery, Xiehe Hospital, Huazhong University of Science and Technology)
Publication Information
BMB Reports / v.47, no.10, 2014 , pp. 552-557 More about this Journal
Abstract
The main purpose of this study was to investigate whether type 3 muscarinic acetylcholine receptor (M3R) dysfunction induced vascular hyperpermeability. Transwell system analysis showed that M3R inhibition by selective antagonist 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and small interfering RNA both increased endothelial permeability. Using coimmunoprecipitation and Western blot assay, we found that M3R inhibition increased VE-cadherin and ${\beta}$-catenin tyrosine phosphorylation without affecting their expression. Using PTP1B siRNA, we found that PTP1B was required for maintaining VE-cadherin and ${\beta}$-catenin protein dephosphorylation. In addition, 4-DAMP suppressed PTP1B activity by reducing cyclic adenosine monophosphate (cAMP), but not protein kinase $C{\alpha}$ ($PKC{\alpha}$). These data indicate that M3R preserves the endothelial barrier function through a mechanism potentially maintaining PTP1B activity, keeping the adherens junction proteins (AJPs) dephosphorylation.
Keywords
${\beta}$-catenin; M3R; PTP1B; VE-cadherin;
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