Functional characterizations of residues Arg-158 and Tyr-170 of the mosquito-larvicidal Bacillus thuringiensis Cry4Ba |
Leetachewa, Somphob
(Bacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University)
Moonsom, Saengduen (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University) Chaisri, Urai (Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University) Khomkhum, Narumol (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University) Yoonim, Nonglak (Faculty of Medical Technology, Western University) Wang, Ping (Department of Entomology, New York State Agricultural Experiment Station, Cornell University) Angsuthanasombat, Chanan (Bacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University) |
1 | Bravo, A., Gill, S. S. and Soberon, M. (2007) Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423-435. DOI ScienceOn |
2 | Ito, T., Bando, H. and Asano, S. (2006) Activation process of the mosquitocidal -endotoxin Cry39A produced by Bacillus thuringiensis subsp. aizawai BUN1-14 and binding property to Anopheles stephensi BBMV. J. Invertebr. Pathol. 93, 29-35. DOI ScienceOn |
3 | Angsuthanasombat, C., Crickmore, N. and Ellar, D. J. (1991) Cytotoxicity of a cloned Bacillus thuringiensis subsp. israelensis CryIVB toxin to an Aedes aegypti cell line. FEMS Microbiol. Lett. 67, 273-276. |
4 | Halstead, S. B. (2008) Dengue virus-mosquito interactions. Annu. Rev. Entomol. 53, 273-291. DOI ScienceOn |
5 | Angsuthanasombat, C., Uawithya, P., Leetachewa, S., Pornwiroon, W., Ounjai, P., Kerdcharoen, T., Katzenmeier, G. R. and Panyim, S. (2004) Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: homology- based 3D model and implications for toxin activity. J. Biochem. Mol. Biol. 37, 304-313. DOI ScienceOn |
6 | Boonserm, P., Davis, P., Ellar, D. J. and Li, J. (2005) Crystal structure of the mosquito-larvicidal toxin Cry4Ba and its biological implications. J. Mol. Biol. 348, 363-382. DOI ScienceOn |
7 | Knowles, B. H. and Ellar, D. J. (1987) Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis -endotoxins with different insect specificity. Biochimica et Biophysica Acta (BBA) - General Subjects 924, 509-518. DOI ScienceOn |
8 | Gerber, D. and Shai, Y. (2000) Insertion and organization within membranes of the -endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant helix 4 peptide. J. Biol. Chem. 275, 23602-23607. DOI ScienceOn |
9 | Leetachewa, S., Katzenmeier, G. and Angsuthanasombat, C. (2006) Novel preparation and characterization of the 4-loop-5 membrane-perturbing peptide from the Bacillus thuringiensis Cry4Ba -endotoxin. J. Biochem. Mol. Biol. 39, 270-277. DOI ScienceOn |
10 | Masson, L., Tabashnik, B. E., Liu, Y. B., Brousseau, R. and Schwartz, J. L. (1999) Helix 4 of the Bacillus thuringiensis Cry1Aa toxin lines the lumen of the ion channel. J. Biol. Chem. 274, 31996-32000. DOI |
11 | Vachon, V., Prefontaine, G., Rang, C., Coux, F., Juteau, M., Schwartz, J. L., Brousseau, R., Frutos, R., Laprade, R. and Masson, L. (2004) Helix 4 mutants of the Bacillus thuringiensis insecticidal toxin Cry1Aa display altered pore-forming abilities. Appl. Environ. Microbiol. 70, 6123-6130. DOI ScienceOn |
12 | Sramala, I., Leetachewa, S., Krittanai, C., Katzenmeier, G., Panyim, S. and Angsuthanasombat, C. (2001) Charged residues screening in helix 4 of the Bacillus thuringiensis Cry4B toxin reveals one critical residue for larvicidal activity. J. Biochem. Mol. Biol. Biophys. 5, 219-215. |
13 | Sramala, I. (2002) Molecuar biophysical studies of transmembrane helices in the pore-forming domain of the Bacillus thuringiensis Cry4B toxin; in: Institute of Molecular Biology and Genetics, pp. 232, Mahidol University, Nakhon Pathom. |
14 | Kanintronkul, Y., Sramala, I., Katzenmeier, G., Panyim, S. and Angsuthanasombat, C. (2003) Specific mutations within the α4-5 loop of the Bacillus thuringiensis Cry4B toxin reveal a crucial role for Asn-166 and Tyr-170. Mol. Biotechnol. 24, 11-20. DOI ScienceOn |
15 | Lehane, M. J. (1997) Peritrophic matrix structure and function. Annu. Rev. Entomol. 42, 525-550. DOI ScienceOn |
16 | Khaokhiew, T., Angsuthanasombat, C. and Promptmas, C. (2009) Correlative effect on the toxicity of three surface- exposed loops in the receptor-binding domain of the Bacillus thuringiensis Cry4Ba toxin. FEMS Microbiol. Lett. 300, 139-145. DOI ScienceOn |
17 | Peters, W. (1992) Peritrophic membranes 1 ed., Springer, Berin. |
18 | Jurat-Fuentes, J. L. and Adang, M. J. (2001) Importance of Cry1 -endotoxin domain II loops for binding specificity in Heliothis virescens (L.). Appl. Environ. Microbiol. 67, 323-329. DOI ScienceOn |
19 | Lee, M. K., You, T. H., Curtiss, A. and Dean, D. H. (1996) Involvement of two amino acid residues in the loop region of Bacillus thuringiensis Cry1Ab toxin in toxicity and binding to Lymantria dispar. Biochem. Biophys. Res. Commun. 229, 139-146. DOI ScienceOn |
20 | Tuntitippawan, T., Boonserm, P., Katzenmeier, G. and Angsuthanasombat, C. (2005) Targeted mutagenesis of loop residues in the receptor-binding domain of the Bacillus thuringiensis Cry4Ba toxin affects larvicidal activity. FEMS Microbiol. Lett. 242, 325-332. DOI ScienceOn |
21 | Likitvivatanavong, S., Aimanova, K. G. and Gill, S. S. (2009) Loop residues of the receptor binding domain of Bacillus thuringiensis Cry11Ba toxin are important for mosquitocidal activity. FEBS Lett. 583, 2021-2030. DOI ScienceOn |
22 | Borovsky, D. and Meola, S. M. (2004) Biochemical and cytoimmunological evidence for the control of Aedes aegypti larval trypsin with Aea-TMOF. Arch. Insect Biochem. Physiol. 55, 124-139. DOI ScienceOn |
23 | Moonsom, S. (2004) Expression and characterization of the cloned domain II and II-III fragments of the Bacillus thurigiensis Cry4Ba larvicidal protein; in: Molecular Genetics and Genetic Engineering, Faculty of Graduate Studies, pp. 133, Mahidol University, Nakhon Pathom. |
24 | Rausell, C., Munoz-Garay, C., Miranda-CassoLuengo, R., Gomez, I., Rudino-Pinera, E., Soberon, M. and Bravo, A. (2004) Tryptophan spectroscopy studies and black lipid bilayer analysis indicate that the oligomeric structure of Cry1Ab toxin from Bacillus thuringiensis is the membrane- insertion intermediate. Biochemistry 43, 166-174. DOI ScienceOn |
25 | Moskalyk, L. A., Oo, M. M. and Jacobs-Lorena, M. (1996) Peritrophic matrix proteins of Anopheles gambiae and Aedes aegypti. Insect Mol. Biol. 5, 261-268. DOI ScienceOn |
26 | Edwards, M. J. and Jacobs-Lorena, M. (2000) Permeability and disruption of the peritrophic matrix and caecal membrane from Aedes aegypti and Anopheles gambiae mosquito larvae. J. Insect. Physiol. 46, 1313-1320. DOI ScienceOn |
27 | Chayaratanasin, P., Moonsom, S., Sakdee, S., Chaisri, U., Katzenmeier, G. and Angsuthanasombat, C. (2007) High level of soluble expression in Escherichia coli and characterisation of the cloned Bacillus thuringiensis Cry4Ba domain III fragment. J. Biochem. Mol. Biol. 40, 58-64. DOI ScienceOn |