Browse > Article
http://dx.doi.org/10.5483/BMBRep.2013.46.7.250

Endothelin-1 enhances the melanogenesis via MITF-GPNMB pathway  

Zhang, Ping (Department of Dermatology, Xijing Hospital, Fourth Military Medical University)
Liu, Wei (Department of Dermatology, Xijing Hospital, Fourth Military Medical University)
Yuan, Xiaoying (Department of Dermatology, The Air Force General Hospital of PLA)
Li, Dongguang (Department of Dermatology, The Air Force General Hospital of PLA)
Gu, Weijie (Department of Dermatology, The Air Force General Hospital of PLA)
Gao, Tianwen (Department of Dermatology, Xijing Hospital, Fourth Military Medical University)
Publication Information
BMB Reports / v.46, no.7, 2013 , pp. 364-369 More about this Journal
Abstract
Endothelin-1 (ET-1) plays an indispensable role in epidermal pigmentation in hyperpigmentary disorders due to a central role in melanogenesis. Nevertheless, precise mechanism involved in ET-1-induced hyperpigmentation is still undefined. Glycoprotein (transmembrane) non-metastatic melanoma protein b (GPNMB) is a key element in melanosome formation. Therefore, we speculated that GPNMB was correlated with ET-1-induced pigmentation. After culturing with ET-1, melanin synthesis was significantly up-regulated, accompanying with increased expression of GPNMB and microphthalmia-associated transcription factor (MITF). Total number of melanosomes and melanin synthesis were sharply reduced via GPNMB-siRNA transfection, indicating ET-1-induced pigmentation by GPNMB-dependent manner. Furthermore, MITF-siRNA transfection strikingly inhibited GPNMB expression and the melanogenesis, and this suppression failed to be alleviated by ET-1 stimulation. All of these results demonstrated that ET-1 can trigger melanogenesis via the MITF-regulated GPNMB pathway. Taken together, these findings will provide a new explanation of how ET-1 induces hyperpigmentation, and possibly supply a new strategy for cosmetic studies.
Keywords
Endothelin-1; GPNMB; Melanogenesis; MITF; Pigment disease;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Levy, C., Khaled, M. and Fisher, D. E. (2006) MITF: master regulator of melanocyte development and melanoma oncogene. Trends Mol. Med. 12, 406-414.   DOI   ScienceOn
2 Dynek, J. N., Chan, S. M., Liu, J., Zha, J., Fairbrother, W. J. and Vucic, D. (2008) Microphthalmia-associated transcription factor is a critical transcriptional regulator of melanoma inhibitor of apoptosis in melanomas. Cancer Res. 68, 3124-3132.   DOI   ScienceOn
3 Zbytek, B., Pfeffer, L. M. and Slominski, A. T. (2006) CRH inhibits NF-${\kappa}B$ signaling in human melanocytes. Peptides 27, 3276-3283.   DOI   ScienceOn
4 Manaka, L., Kadono, S., Kawashima, M., Kobayashi, T. and Imokawa, G. (2001) The mechanism of hyperpigmentation in seborrhoeic keratosis involves the high expression of endothelin-converting enzyme-1alpha and TNF-alpha, which stimulate secretion of endothelin 1. Br. J. Dermatol. 145, 895-903.   DOI   ScienceOn
5 Nakajima, H., Wakabayashi, Y., Wakamatsu, K. and Imokawa, G. (2011) An extract of withania somnifera attenuates endothelin-1-stimulated pigmentation in human epidermal equivalents through the interruption of pkc activity within melanocytes. Phytother. Res. 25, 1398-1411.
6 Chi, A., Valencia, J. C., Hu, Z. Z., Watabe, H., Yamaguchi, H., Mangini, N. J., Huang, H., Canfield, V. A., Cheng, K. C. and Yang, F. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. J. Proteome Res. 5, 3135-3144.   DOI   ScienceOn
7 Stanisz, H., Stark, A., Kilch, T., Schwarz, E. C., Müller, C. S. L., Peinelt, C., Hoth, M., Niemeyer, B. A., Vogt, T. and Bogeski, I. (2012) ORAI1 $Ca^{2+}$ channels control endothelin- 1-induced mitogenesis and melanogenesis in primary human melanocytes. J. Invest. Dermatol. 132, 1443-1451.   DOI   ScienceOn
8 Hirobe, T. (2011) How are proliferation and differentiation of melanocytes regulated? Pigment Cell Melanoma Res. 24, 462-478.   DOI   ScienceOn
9 Vachtenheim, J. and Borovanský, J. (2010) "Transcription physiology" of pigment formation in melanocytes: central role of MITF. Exp. Dermatol. 19, 617-627.   DOI   ScienceOn
10 Hoek, K. S., Schlegel, N. C., Eichhoff, O. M., Widmer, D. S., Praetorius, C., Einarsson, S. O., Valgeirsdottir, S., Bergsteinsdottir, K., Schepsky, A. and Dummer, R. (2008) Novel MITF targets identified using a two-step DNA microarray strategy. Pigment Cell Melanoma Res. 21, 665-676.   DOI   ScienceOn
11 Loftus, S. K., Antonellis, A., Matera, I., Renaud, G., Baxter, L. L., Reid, D., Wolfsberg, T. G., Chen, Y., Wang, C. W. and Prasad, M. K. (2009) Gpnmb is a melanoblast-expressed, MITF-dependent gene. Pigment Cell Melanoma Res. 22, 99-110.   DOI   ScienceOn
12 Weterman, M. A. J., Ajubi, N., van Dinter, I. M. R., Degen, W. G. J., van Muijen, G. N. P., Ruiter, D. J. and Bloemers, H. P. J. (1995) nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts. Int. J. Cancer 60, 73-81.   DOI   ScienceOn
13 Hoashi, T., Sato, S., Yamaguchi, Y., Passeron, T., Tamaki, K. and Hearing, V. J. (2010) Glycoprotein nonmetastatic melanoma protein b, a melanocytic cell marker, is a melanosome-specific and proteolytically released protein. FASEB J. 24, 1616-1629.   DOI   ScienceOn
14 Tomihari, M., Hwang, S. H., Chung, J. S., Cruz, Jr. P. D. and Ariizumi, K. (2009) Gpnmb is a melanosome-associated glycoprotein that contributes to melanocyte/keratinocyte adhesion in a RGD-dependent fashion. Exp. Dermatol. 18, 586-595.   DOI   ScienceOn
15 Zhang, P., Liu, W., Zhu, C. S., Yuan, X. Y., Li, D. G., Gu, W. J., Ma, H. M., Xie, X. and Gao, T. W. (2012) Silencing of GPNMB by siRNA inhibits the formation of melanosomes in melanocytes in a MITF-Independent fashion. PloS One 7, e42955.   DOI
16 Hafner, C. and Vogt, T. (2008) Seborrheic keratosis. J. Dtsch. Dermatol. Ges. 6, 664-677.   DOI   ScienceOn
17 Lim, C. (2006) Seborrhoeic keratoses with associated lesions: a retrospective analysis of 85 lesions. Australas. J. Dermatol. 47, 109-113.   DOI   ScienceOn
18 Abreu Velez, A., Klein, A. and Howard, M. (2011) Overexpression of linker for activated T cells, cyclooxygenase-2, CD1a, CD68 and my eloid/histiocyte antigen in an inflamed seborrheic keratosis. North. Am. J. Med. Sci. 3, 161-163.
19 Petersson, F., Ivan, D., Kazakov, D. V., Michal, M. and Prieto, V. G. (2009) Pigmented paget disease-a diagnostic pitfall mimicking melanoma. Am. J. Dermatopathol. 31, 223-226.   DOI   ScienceOn
20 Kawaguchi, Y., Mori, N. and Nakayama, A. (2001) Kit+ melanocytes seem to contribute to melanocyte proliferation after UV exposure as precursor cells. J. Invest. Dermatol. 116, 920-925.   DOI   ScienceOn
21 Singh, S. K., Kurfurst, R., Nizard, C., Schnebert, S., Perrier, E. and Tobin, D. J. (2010) Melanin transfer in human skin cells is mediated by filopodia-a model for homotypic and heterotypic lysosome-related organelle transfer. FASEB J. 24, 3756-3769.   DOI   ScienceOn
22 Schallreuter, K. U., Kothari, S., Chavan, B. and Spencer, J. D. (2008) Regulation of melanogenesis-controversies and new concepts. Exp. Dermatol. 17, 395-404.   DOI   ScienceOn
23 Imokawa, G., Kobayashi, T., Miyagishi, M., Higashi, K. and Yada, Y. (1997) The role of endothelin-1 in epidermal hyperpigmentation and signaling mechanisms of mitogenesis and melanogenesis. Pigment. Cell Res. 10, 218-228.   DOI   ScienceOn
24 Yanagisawa, M., Kurihara, H., Kimura, S., Tomobe, Y., Kobayashi, M., Mitsui, Y., Yazaki, Y., Goto, K. and Masaki, T. (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332, 411-415.   DOI   ScienceOn
25 Hemesath, T. J., Price, E. R., Takemoto, C., Badalian, T. and Fisher, D. E. (1998) MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes. Nature 391, 298-301.   DOI   ScienceOn
26 Hachiya, A., Kobayashi, A., Yoshida, Y., Kitahara, T., Takema, Y. and Imokawa, G. (2004) Biphasic expression of two paracrine melanogenic cytokines, stem cell factor and endothelin-1, in ultraviolet B-induced human melanogenesis. Am. J. Pathol. 165, 2099-2109.   DOI   ScienceOn
27 Sato-Jin, K., Nishimura, E. K., Akasaka, E., Huber, W., Nakano, H., Miller, A., Du, J., Wu, M., Hanada, K. and Sawamura, D. (2008) Epistatic connections between microphthalmia-associated transcription factor and endothelin signaling in Waardenburg syndrome and other pigmentary disorders. FASEB J. 22, 1155-1168.   DOI