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http://dx.doi.org/10.5483/BMBRep.2013.46.4.160

Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells  

Lee, Young-Rae (Department of Oral Biochemistry, School of Dentistry, Wonkwang University)
Noh, Eun-Mi (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School)
Han, Ji-Hey (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School)
Kim, Jeong-Mi (Department of Oral Biochemistry, School of Dentistry, Wonkwang University)
Hwang, Bo-Mi (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School)
Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Sung-Ho (Department of Companion and Laboratory Animal Science, Kongju National University)
Jung, Sung Hoo (Division of Breast Thyroid Surgery, Department of Surgery, Chonbuk National University Medical School)
Youn, Hyun Jo (Division of Breast Thyroid Surgery, Department of Surgery, Chonbuk National University Medical School)
Chung, Eun Yong (Department of Anestheiology and Pain Medicine, St. Mary's Hospital, College of Medicine, The Catholic University of Korea)
Kim, Jong-Suk (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School)
Publication Information
BMB Reports / v.46, no.4, 2013 , pp. 201-206 More about this Journal
Abstract
Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is an isothiocyanate found in some cruciferous vegetables, especially broccoli. Sulforaphane has been shown to display anti-cancer properties against various cancer cell lines. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. In this study, we investigated the effect of sulforaphane on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. TPA-induced MMP-9 expression and cell invasion were decreased by sulforaphane treatment. TPA substantially increased NF-${\kappa}B$ and AP-1 DNA binding activity. Pre-treatment with sulforaphane inhibited TPA-stimulated NF-${\kappa}B$ binding activity, but not AP-1 binding activity. In addition, we found that sulforaphane suppressed NF-${\kappa}B$ activation, by inhibiting phosphorylation of $I{\kappa}B $ in TPA-treated MCF-7 cells. In this study, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by sulforaphane was mediated by the suppression of the NF-${\kappa}B$ pathway in MCF-7 cells.
Keywords
Invasion; MCF-7; MMP-9; NF-${\kappa}B$; Sulforaphane;
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