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http://dx.doi.org/10.5483/BMBRep.2013.46.2.141

Analysis and characterization of the functional TGFβ receptors required for BMP6-induced osteogenic differentiation of mesenchymal progenitor cells  

Zhang, Yan (Oral Diseases and Biomedical Research Center, the Affiliated Hospital of Stomatology, Chongqing Medical University)
Zhang, De-Ying (Institute of Pediatric Research, Children's Hospital of Chongqing Medical University)
Zhao, Yan-Fang (Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University)
Wang, Jin (Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University)
He, Juan-Wen (Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University)
Luo, Jinyong (Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University)
Publication Information
BMB Reports / v.46, no.2, 2013 , pp. 107-112 More about this Journal
Abstract
Although BMP6 is highly capable of inducing osteogenic differentiation of mesenchymal progenitor cells (MPCs), the molecular mechanism involved remains to be fully elucidated. Using dominant negative (dn) mutant form of type I and type II $TGF{\beta}$ receptors, we demonstrated that three dn-type I receptors (dnALK2, dnALK3, dnALK6), and three dn-type II receptors (dnBMPRII, dnActRII, dnActRIIB), effectively diminished BMP6-induced osteogenic differentiation of MPCs. These findings suggested that ALK2, ALK3, ALK6, BMPRII, ActRII and ActRIIB are essential for BMP6-induced osteogenic differentiation of MPCs. However, MPCs in this study do not express ActRIIB. Moreover, RNA interference of ALK2, ALK3, ALK6, BMPRII and ActRII inhibited BMP6-induced osteogenic differentiation in MPCs. Our results strongly suggested that BMP6-induced osteogenic differentiation of MPCs is mediated by its functional $TGF{\beta}$ receptors including ALK2, ALK3, ALK6, BMPRII, and ActRII.
Keywords
BMP6; Bone morphogenetic proteins; Mesenchymal progenitor cells; Osteogenic differentiation; Transforming growth factor ${\beta}$ receptor;
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