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http://dx.doi.org/10.5483/BMBRep.2013.46.12.082

Identification of anti-adipogenic proteins in adult bovine serum suppressing 3T3-L1 preadipocyte differentiation  

Park, Jeongho (School of Biotechnology, Yeungnam University)
Park, Jihyun (School of Biotechnology, Yeungnam University)
Nahm, Sang-Soep (College of Veterinary Medicine Konkuk University)
Choi, Inho (School of Biotechnology, Yeungnam University)
Kim, Jihoe (School of Biotechnology, Yeungnam University)
Publication Information
BMB Reports / v.46, no.12, 2013 , pp. 582-587 More about this Journal
Abstract
Adipocyte differentiation is a complex developmental process forming adipocytes from various precursor cells. The murine 3T3-L1 preadipocyte cell line has been most frequently used in the studies of adipocyte differentiation. Differentiation of 3T3-L1 preadipocytes includes a medium containing fetal bovine serum (FBS) with hormonal induction. In this study, we observed that differentiation medium containing adult bovine serum (ABS) instead of FBS did not support differentiation of preadipocytes. Impaired adipocyte differentiation was due to the presence of a serum protein factor in ABS that suppresses differentiation of preadipocytes. Using a proteomic analysis, alpha-2-macroglobulin and paraoxonase/arylesterase 1, which were previously shown to suppress differentiation of preadipocytes, were identified as anti-adipogenic proteins. Although their functional mechanisms have not yet been elucidated, the anti-adipogenic effects of these proteins are discussed.
Keywords
Adipocyte; Alpha-2-macroglobulin; Differentiation; Paraoxonase; Serum;
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1 Lowe, C. E., O'Rahilly, S. and Rochford, J. J. (2011) Adipogenesis at a glance. J. Cell Sci. 124, 2681-2686.   DOI   ScienceOn
2 Sarjeant, K. and Stephens, J. M. (2012) Adipogenesis. Cold Spring Harb. Perspect. Biol. 4, a008417.
3 Poulos, S. P., Dodson, M. V. and Hausman, G. J. (2010) Cell line models for differentiation: preadipocytes and adipocytes. Exp. Biol. Med. 235, 1185-1193.   DOI   ScienceOn
4 Kuri-Harcuch, W. and Green, H. (1978) Adipose conversion of 3T3 cells depends on a serum factor. Proc. Natl. Acad. Sci. U. S. A. 75, 6107-6109.   DOI   ScienceOn
5 Justesen, J., Stenderup, K., Eriksen, E. F. and Kassem, M. (2002) Maintenance of osteoblastic and adipocytic differentiation potential with age and osteoporosis in human marrow stromal cell cultures. Calcif. Tissue. Int. 71, 36-44.   DOI   ScienceOn
6 Gerard, C., Blouin, K., Tchernof, A. and Doillon, C. J. (2008) Adipogenesis in nonadherent and adherent bone marrow stem cells grown in fibrin gel and in the presence of adult plasma. Cells Tissues. Organs. 187, 186-198.   DOI   ScienceOn
7 Lee, D. M., Bajracharya, P., Lee, E. J., Kim, J. E., Lee, H. J., Chun, T., Kim, J., Cho, K. H., Chang, J., Hong, S. and Choi, I. (2011) Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation. In Vitro Cell Dev. Biol. Anim. 47, 438-444.   DOI   ScienceOn
8 Kim, J., Kim, M., Nahm, S., Lee, D., Pokharel, S. and Choi, I. (2011) Characterization of gender-pecific bovine serum. Anmal Cells Syst. 15, 147-154.   DOI
9 Choi, K. L., Wang, Y., Tse, C. A., Lam, K. S., Cooper, G. J. and Xu, A. (2004) Proteomic analysis of adipocyte differentiation: Evidence that alpha2 macroglobulin is involved in the adipose conversion of 3T3 L1 preadipocytes. Proteomics 4, 1840-1848.   DOI   ScienceOn
10 Eckerson, H. W., Wyte, C. M. and La Du, B. N. (1983) The human serum paraoxonase/arylesterase polymorphism. Am. J. Hum. Genet. 35, 1126-1138.
11 Rosenblat, M., Coleman, R., Reddy, S. T. and Aviram, M. (2009) Paraoxonase 2 attenuates macrophage triglyceride accumulation via inhibition of diacylglycerol acyltransferase 1. J. Lipid. Res. 50, 870-879.
12 Seres, I., Bajnok, L., Harangi, M., Sztanek, F., Koncsos, P. and Paragh, G. (2010) Alteration of PON1 activity in adult and childhood obesity and its relation to adipokine levels. Adv. Exp. Med. Biol. 660, 129-142.   DOI   ScienceOn
13 Miyamoto, T., Takahashi, Y., Oohashi, T., Sato, K. and Oikawa, S. (2005) Bovine paraoxonase 1 activities in serum and distribution in lipoproteins. J. Vet. Med Sci. 67, 243-248.   DOI   ScienceOn
14 Wollenberg, G. K., LaMarre, J., Semple, E., Farber, E., Gauldie, J. and Hayes, M. A. (1991) Counteracting effects of dexamethasone and alpha 2-macroglobulin on inhibition of proliferation of normal and neoplastic rat hepatocytes by transforming growth factors-beta type 1 and type 2. Int. J. Cancer 47, 311-316.   DOI
15 Bellei, E., Bergamini, S., Monari, E., Fantoni, L. I., Cuoghi, A., Ozben, T. and Tomasi, A. (2011) High-abundance proteins depletion for serum proteomic analysis: concomitant removal of non-targeted proteins. Amino Acids 40, 145-156.   DOI
16 Atha, D. H. and Ingham, K. C. (1981) Mechanism of precipitation of proteins by polyethylene glycols. Analysis in terms of excluded volume. J. Biol. Chem. 256, 12108-12117.
17 Bode, J. G., Albrecht, U., Haussinger, D., Heinrich, P. C. and Schaper, F. (2012) Hepatic acute phase proteins--regulation by IL-6- and IL-1-type cytokines involving STAT3 and its crosstalk with NF-kappaB-dependent signaling. Eur. J. Cell Biol. 91, 496-505.   DOI   ScienceOn
18 LaMarre, J., Wollenberg, G. K., Gonias, S. L. and Hayes, M. A. (1991) Reaction of alpha 2-macroglobulin with plasmin increases binding of transforming growth factors-beta 1 and beta 2. Biochim. Biophys. Acta. 1091, 197-204.   DOI   ScienceOn
19 LaMarre, J., Hayes, M. A., Wollenberg, G. K., Hussaini, I., Hall, S. W. and Gonias, S. L. (1991) An alpha 2-macroglobulin receptor-dependent mechanism for the plasma clearance of transforming growth factor-beta 1 in mice. J. Clin. Invest. 87, 39-44.   DOI   ScienceOn
20 Patel, Y. M. and Lane, M. D. (2000) Mitotic clonal expansion during preadipocyte differentiation: calpain-mediated turnover of p27. J. Biol. Chem. 275, 17653-17660.   DOI   ScienceOn
21 Ferretti, G., Bacchetti, T., Masciangelo, S. and Bicchiega, V. (2010) HDL-paraoxonase and membrane lipid peroxidation: a comparison between healthy and obese subjects. Obesity 18, 1079-1084.   DOI   ScienceOn
22 Patel, Y. M. and Lane, M. D. (1999) Role of calpain in adipocyte differentiation. Proc. Natl. Acad. Sci. U. S. A. 96, 1279-1284.   DOI   ScienceOn
23 Wang, X., Huang, J., Zhao, L., Wang, C., Ju, Z., Li, Q., Qi, C., Zhang, Y., Zhang, Z., Zhang, W., Hou, M., Yuan, J. and Zhong, J. (2012) The exon 29 c.3535A>T in the alpha-2-macroglobulin gene causing aberrant splice variants is associated with mastitis in dairy cattle. Immunogenetics 64, 807-816.   DOI   ScienceOn
24 van Himbergen, T. M., van Tits, L. J., Roest, M. and Stalenhoef, A. F. (2006) The story of PON1: how an organophosphate-hydrolysing enzyme is becoming a player in cardiovascular medicine. Neth. J. Med. 64, 34-38.
25 Ferretti, G., Bacchetti, T., Moroni, C., Savino, S., Liuzzi, A., Balzola, F. and Bicchiega, V. (2005) Paraoxonase activity in high-density lipoproteins: a comparison between healthy and obese females. J. Clin. Endocrinol. Metab. 90, 1728-1733.   DOI
26 Schroder, K., Wandzioch, K., Helmcke, I. and Brandes, R. P. (2009) Nox4 acts as a switch between differentiation and proliferation in preadipocytes. Arterioscler. Thromb. Vasc. Biol. 29, 239-245.   DOI   ScienceOn
27 Lee, H., Lee, Y. J., Choi, H., Ko, E. H. and Kim, J. W. (2009) Reactive oxygen species facilitate adipocyte differentiation by accelerating mitotic clonal expansion. J. Biol. Chem. 284, 10601-10609.   DOI   ScienceOn
28 Jeong, J., Ha, T. S. and Kim, J. (2011) Protection of aquo/hydroxocobalamin from reduced glutathione by a B12 trafficking chaperone. BMB Rep. 44, 170-175.   DOI   ScienceOn
29 Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.   DOI   ScienceOn
30 Jeong, J., Park, J., Lee, D. Y. and Kim, J. (2013) C-terminal truncation of a bovine B(12) trafficking chaperone enhances the sensitivity of the glutathione-regulated thermostability. BMB Rep. 46, 169-174.   과학기술학회마을   DOI   ScienceOn
31 Jung, S. R., Song, N. J., Hwang, H. S., An, J. J., Cho, Y. J., Kweon, H. Y., Kang, S. W., Lee, K. G., Yoon, K., Kim, B. J., Nho, C. W., Choi, S. Y. and Park, K. W. (2011) Silk peptides inhibit adipocyte differentiation through modulation of the Notch pathway in C3H10T1/2 cells. Nutr. Res. 31, 723-730.   DOI   ScienceOn
32 Ganrot, P. O. (1966) Determination of alpha-2-macroglobulin as trypsin-protein esterase. Clin. Chim. Acta. 14, 493-501.   DOI   ScienceOn