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http://dx.doi.org/10.5483/BMBRep.2013.46.10.015

Differential expression of αB-crystallin causes maturation-dependent susceptibility of oligodendrocytes to oxidative stress  

Kim, Ji Young (Department of Anatomy, Chungbuk National University School of Medicine)
Lee, Eun Young (Department of Anatomy, Chungbuk National University School of Medicine)
Sohn, Hyun Joon (Department of Anatomy, Chungbuk National University School of Medicine)
Kim, Si Wook (Department of Thoracic and Cardiovascular Surgery, Chungbuk National University School of Medicine)
Kim, Chan Hyung (Department of Pharmacology, Chungbuk National University School of Medicine)
Ahn, Hee Yul (Department of Pharmacology, Chungbuk National University School of Medicine)
Kim, Dong Woon (Department of Anatomy, Chungnam National University School of Medicine)
Cho, Sa Sun (Department of Anatomy, Jeju National University School of Medicine)
Seo, Je Hoon (Department of Anatomy, Chungbuk National University School of Medicine)
Publication Information
BMB Reports / v.46, no.10, 2013 , pp. 501-506 More about this Journal
Abstract
Oligodendrocyte precursor cells (OPCs) are most susceptible to oxidative stress in the brain. However, the cause of differences in susceptibility to oxidative stress between OPCs and mature oligodendrocytes (mOLs) remains unclear. Recently, we identified in vivo that ${\alpha}B$-crystallin (aBC) is expressed in mOLs but not in OPCs. Therefore, we examined in the present study whether aBC expression could affect cell survival under oxidative stress induced by hydrogen peroxide using primary cultures of OPCs and mOLs from neonatal rat brains. Expression of aBC was greater in mOLs than in OPCs, and the survival rate of mOLs was significantly higher than that of OPCs under oxidative stress. Suppression of aBC by siRNA transfection resulted in a decrease in the survival rate of mOLs under oxidative stress. These data suggest that higher susceptibility of OPCs than mOLs to oxidative stress is due, at least in part, to low levels of aBC expression.
Keywords
${\alpha}B$-crystallin; Cell survival; Oligodendrocyte; Oligoden-drocyte precursor cell; Oxidative stress;
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