[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5483/BMBRep.2013.46.10.028

Anticoagulant activities of piperlonguminine in vitro and in vivo

Lee, Wonhwa (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University)
Yoo, Hayoung (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University)
Ku, Sae-Kwang (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University)
Kim, Jeong Ah (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University)
Bae, Jong-Sup (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University)

Publication Information

BMB Reports / v.46, no.10, 2013 , pp. 484-489 More about this Journal

Abstract

Piperlonguminine (PL), an important component of Piper longum fruits, is known to exhibit anti-hyperlipidemic, antiplatelet and anti-melanogenic activities. Here, the anticoagulant activities of PL were examined by monitoring activated-partial-thromboplastin-time (aPTT), prothrombin-time (PT), and the activities of thrombin and activated factor X (FXa). The effects of PL on the expressions of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were also tested in tumor necrosis factor- ${\alpha}$ (TNF- ${\alpha}$ ) activated HUVECs. The results showed that PL prolonged aPTT and PT significantly and inhibited the activities of thrombin and FXa. PL inhibited the generation of thrombin and FXa in HUVECs. In accordance with these anticoagulant activities, PL prolonged in vivo bleeding time and inhibited TNF- ${\alpha}$ induced PAI-1 production. Furthermore, PAI-1/t-PA ratio was significantly decreased by PL. Collectively, our results suggest that PL possesses antithrombotic activities and that the current study could provide bases for the development of new anticoagulant agents.

Keywords

Coagulation cascade; Endothelium; Fibrinolysis; Piperlonguminine;

Citations & Related Records

Times Cited By KSCI : 3 (Citation Analysis)

Reference
Cited By KSCI

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