Browse > Article
http://dx.doi.org/10.5483/BMBRep.2012.45.9.048

Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells  

Kim, Dong-Bum (Department of Microbiology, College of Medicine, Hallym University)
Park, Min-Chul (Department of Microbiology, College of Medicine, Hallym University)
Park, Byoung-Kwon (Department of Microbiology, College of Medicine, Hallym University)
Kwon, Sang-Hoon (Center for Medical Science Research, College of Medicine, Hallym University)
Choi, Joon-Ho (Department of Food Science and Biotechnology, College of Life Science and Natural Resources, Wonkwang University)
Kim, Hyun-Jong (Experimental Animal Center, Hallym University)
Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University)
Park, Jin-Seu (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University)
Lee, Young-Hee (Department of Biochemistry, College of Natural Science, Chungbuk National University)
Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
Publication Information
BMB Reports / v.45, no.9, 2012 , pp. 538-543 More about this Journal
Abstract
We evaluated the expression of the costimulatory molecules CD80 and CD83 and major histocompatibility (MHC) class II induced by 2,4-dinitrofluorobenzene (DNFB) in the RAW 264.7 macrophage cell line. In contrast to the previously reported effect of DNFB on dendritic cells, CD86 expression did not change. Furthermore, we observed that the CD83 expression level transiently increased and then decreased. Induction of CD80 and MHC class II molecule expression and a decrease in CD83 expression by DNFB in vitro were also confirmed in splenocytes of BALB/c and NC/Nga mice. However, DNFB did not influence CD83 expression in peritoneal $CD11b^+$ cells from BALB/c or NC/Nga mice. Detailed in vivo experiments and further studies on the possible contribution of $CD11b^+$ cells to induce atopic dermatitis (AD) would be helpful to attain a better understanding of AD pathogenesis.
Keywords
Atopic dermatitis; Costimulatory molecules; DNFB; Macrophages; NC/Nga mice;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 An, S., Kim, S., Huh, Y., Lee, T. R., Kim, H. K., Park, K. L. and Eun, H. C. (2009) Expression of surface markers on the human monocytic leukaemia cell line, THP-1, as indicators for the sensitizing potential of chemicals. Contact Dermatitis 60, 185-192.   DOI   ScienceOn
2 Kim, D., Jung, J., Lee, Y. and Kwon, H. J. (2011) Novel immunostimulatory phosphodiester oligodeoxynucleotides with CpT sequences instead of CpG motifs. Mol. Immunol. 48, 1494-1504.   DOI   ScienceOn
3 Matos, T. J., Duarte, C. B., Goncalo, M. and Lopes, M. C. (2005) DNFB activates MAPKs and upregulates CD40 in skin-derived dendritic cells. J. Dermatol. Sci. 39, 113-123.   DOI   ScienceOn
4 Vital, A. L., Goncalo, M., Cruz, M. T., Figueiredo, A., Duarte, C. B. and Celeste Lopes, M. (2004) The sensitizers nickel sulfate and 2,4-dinitrofluorobenzene increase CD40 and IL-12 receptor expression in a fetal skin dendritic cell line. Biosci. Rep. 24, 191-202.   DOI   ScienceOn
5 Dupasquier, M., Stoitzner, P., van Oudenaren, A., Romani, N. and Leenen, P. J. (2004) Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis. J. Invest. Dermatol. 123, 876-879.   DOI   ScienceOn
6 Tuckermann, J. P., Kleiman, A., Moriggl, R., Spanbroek, R., Neumann, A., Illing, A., Clausen, B. E., Stride, B., Förster, I., Habenicht, A. J., Reichardt, H. M., Tronche, F., Schmid, W. and Schütz, G. (2007) Macrophages and neutrophils are the targets for immune suppression by glucocorticoids in contact allergy. J. Clin. Invest. 117, 1381-1390.   DOI   ScienceOn
7 Kim, D., Kim, J., Kwon, S., Kim, Y. J., Lee, S., Lee, Y., Seo, J. N., Park, C. S., Park, K. L. and Kwon, H. J. (2008) Regulation of macrophage inflammatory protein-2 gene expression in response to 2,4-dinitrofluorobenzene in RAW 264.7 cells. BMB Rep. 41, 316-321.   DOI   ScienceOn
8 Kim, D., Kim, Y. J., Seo, J. N., Kim, J., Lee, Y,, Park, C. S., Kim, D. W., Kim, D. S. and Kwon, H. J. (2009) 2,4-Dinitrofluorobenzene modifies cellular proteins and incuces macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells. Immunol. Invest. 38, 132-152.   DOI   ScienceOn
9 Kim, Y. J., Kim, D., Lee, Y., Choi, S. Y., Park, J., Lee, S. Y., Park, J. W. and Kwon, H. J. (2009) Effects of nanoparticulate saponin-platinum conjugates on 2,4-dinitrofluorobenzene- induced macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells. BMB Rep. 42, 304-309.   DOI   ScienceOn
10 Nagai, H., Matsuo, A., Hiyama, H., Inagaki, N. and Kawada, K. (1997) Immunoglobulin E production in mice by means of contact sensitization with a simple chemical, hapten. J. Allergy Clin. Immunol. 100, S39-44.   DOI   ScienceOn
11 Tomimori, Y., Tanaka, Y., Goto, M. and Fukuda, Y. (2005) Repeated topical challenge with chemical antigen elicits sustained dermatitis in NC/Nga mice in specific-pathogen- free condition. J. Invest. Dermatol. 124, 119-124.   DOI   ScienceOn
12 Enk, A. H. (1997) Allergic contact dermatitis: understanding the immune response and potential for targeted therapy using cytokines. Mol. Med. Today 3, 423-428.   DOI   ScienceOn
13 Coutant, K. D., de Fraissinette, A. B., Cordier, A. and Ulrich, P. (1999) Modulation of the activity of human monocyte-derived dendritic cells by chemical haptens, a metal allergen, and a staphylococcal superantigen. Toxicol. Sci. 52, 189-198.   DOI   ScienceOn
14 Kaplan, D. H. (2010) In vivo function of Langerhans cells and dermal dendritic cells. Trends Immunol. 31, 446-451.   DOI   ScienceOn
15 Tuschl, H. and Kovac, R. (2001) Langerhans cells and immature dendritic cells as model systems for screening of skin sensitizers. Toxicol. In Vitro 15, 327-331.   DOI   ScienceOn
16 Pepin, E., Goutet, M. and Ban, M. (2007) Murine bone marrow-derived dendritic cells as a potential in vitro model for predictive identification of chemical sensitizers. Toxicol. Lett. 175, 89-101.   DOI   ScienceOn
17 Akdis, C. A., Akdis, M., Trautmann, A. and Blaser, K. (2000) Immune regulation in atopic dermatitis. Curr. Opin. Immunol. 12, 641-646.   DOI   ScienceOn
18 Herouet, C., Cottin, M., LeClaire, J., Enk, A. and Rousset, F. (2000) Contact sensitizers specifically increase MHC class II expression on murine immature dendritic cells. In Vitr. Mol. Toxicol. 13, 113-123.   DOI   ScienceOn
19 Matos, T. J., Duarte, C. B., Goncalo, M. and Lopes, M. C. (2005) Role of oxidative stress in ERK and p38 MAPK activation induced by the chemical sensitizer DNFB in a fetal skin dendritic cell line. Immunol. Cell Biol. 83, 607-614.   DOI   ScienceOn
20 Kitagaki, H., Ono, N., Hayakawa, K., Kitazawa, T., Watanabe, K. and Shiohara, T. (1997) Repeated elicitation of contact hypersensitivity induces a shift in cutaneous cytokine milieu from a T helper cell type 1 to a T helper cell type 2 profile. J. Immunol. 159, 2484-2491.
21 Kitagaki, H., Fujisawa, S., Watanabe, K., Hayakawa, K. and Shiohara, T. (1995) Immediate-type hypersensitivity response followed by a late reaction is induced by repeated epicutaneous application of contact sensitizing agents in mice. J. Invest. Dermatol. 105, 749-755.   DOI   ScienceOn
22 Leung, D. Y. (1997) Atopic dermatitis: immunobiology and treatment with immune modulators. Clin. Exp. Immunol. 107(Suppl), 25-30.
23 Rudikoff, D. and Lebwohl, M. (1998) Atopic dermatitis. Lancet 351, 1715-1721.   DOI   ScienceOn
24 Novak, N., Bieber, T. and Leung, D. Y. (2003) Immune mechanisms leading to atopic dermatitis. J. Allergy Clin. Immunol. 112(Suppl), S128-139.   DOI   ScienceOn
25 Vestergaard, C., Yoneyama, H., Murai, M., Nakamura, K., Tamaki, K., Terashima, Y., Imai, T., Yoshie, O., Irimura, T., Mizutani, H. and Matsushima, K. (1999) Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis-like lesions. J. Clin. Invest. 104, 1097-1105.   DOI   ScienceOn
26 Ahmed, A. R. and Blose, D. A. (1983) Delayed-type hypersensitivity skin testing. A review. Arch. Dermatol. 119, 934-945.   DOI