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http://dx.doi.org/10.5483/BMBRep.2012.45.9.104

Parkin induces apoptotic cell death in TNF-α-treated cervical cancer cells  

Lee, Kyung-Hong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Lee, Min-Ho (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Kang, Yeo-Wool (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Rhee, Ki-Jong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Kim, Tae-Ue (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Kim, Yoon-Suk (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Publication Information
BMB Reports / v.45, no.9, 2012 , pp. 526-531 More about this Journal
Abstract
Many malignant tumors become resistant to tumor necrosis factor-alpha (TNF-${\alpha}$)-induced cell death during carcinogenesis. In the present study, we examined whether parkin acts as a tumor suppressor in HeLa cells, a human cervical cancer cell line resistant to TNF-${\alpha}$-induced cell death. TNF-${\alpha}$-treatment alone did not affect HeLa cell viability. However, expression of parkin restored TNF-${\alpha}$-induced apoptosis in HeLa cells. Increased cell death was due to the activation of the apoptotic pathway. Expression of parkin in TNF-${\alpha}$-treated HeLa cells stimulated cleavage of the pro-apoptotic proteins caspase-8, -9, -3, -7 and poly ADP ribose polymerase (PARP). In addition, parkin expression resulted in decreased expression of the caspase inhibitory protein, survivin. These results suggest that parkin acts as a tumor suppressor in human cervical cancer cells by modulating survivin expression and caspase activity. We propose that this pathway is a novel molecular mechanism by which parkin functions as a tumor suppressor.
Keywords
Apoptosis; Caspase; Cervical cancer; Parkin; TNF-${\alpha}$;
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