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http://dx.doi.org/10.5483/BMBRep.2012.45.8.033

MicroRNA let-7c inhibits Bcl-xl expression and regulates ox-LDL-induced endothelial apoptosis  

Qin, Bing (Department of Neurology, Xiangya Hospital, Central South University)
Xiao, Bo (Department of Neurology, Xiangya Hospital, Central South University)
Liang, Desheng (State Key Laboratory of Medical Genetics, Central South University)
Li, Ye (Department of Neurology, Xiangya Hospital, Central South University)
Jiang, Ting (Department of Neurology, Xiangya Hospital, Central South University)
Yang, Huan (Department of Neurology, Xiangya Hospital, Central South University)
Publication Information
BMB Reports / v.45, no.8, 2012 , pp. 464-469 More about this Journal
Abstract
Endothelial cells (ECs) apoptosis induced by oxidized low-density lipoprotein (ox-LDL) is thought to play a critical role in atherosclerosis. MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. MiRNA let-7 family is known to be involved in the regulation of cell apoptosis. However, the function of let-7 in ox-LDL induced ECs apoptosis and atherosclerosis is still unknown. Here, we show that let-7c expression was markedly up-regulated in ox-LDL induced apoptotic human umbilical cord vein endothelial cells (HUVECs). Let-7c over-expression enhanced apoptosis in ECs whereas inhibition of let-7c could partly alleviate apoptotic cell death mediated by ox-LDL. Searching for how let-7c affected apoptosis, we discovered that antiapoptotic protein Bcl-xl was a direct target of let-7c in ECs. Our data suggest that let-7c contributes to endothelial apoptosis through suppression of Bcl-xl.
Keywords
Apoptosis; Atherosclerosis; Bcl-xl; HUVECs; MicroRNA;
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