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http://dx.doi.org/10.5483/BMBRep.2012.45.6.276

Anti-fibrotic effects of L-2-oxothiazolidine-4-carboxylic acid via modulation of nuclear factor erythroid 2-related factor 2 in rats  

Kim, In-Hee (Department of Internal Medicine, Chonbuk National University Medical School and Hospital)
Kim, Dae-Ghon (Department of Internal Medicine, Chonbuk National University Medical School and Hospital)
Hao, Peipei (Research Institute of Clinical Medicine, Chonbuk National University Medical School and Hospital)
Wang, Yunpeng (Research Institute of Clinical Medicine, Chonbuk National University Medical School and Hospital)
Kim, Seong-Hun (Department of Internal Medicine, Chonbuk National University Medical School and Hospital)
Kim, Sang-Wook (Department of Internal Medicine, Chonbuk National University Medical School and Hospital)
Lee, Seung-Ok (Department of Internal Medicine, Chonbuk National University Medical School and Hospital)
Lee, Soo-Teik (Department of Internal Medicine, Chonbuk National University Medical School and Hospital)
Publication Information
BMB Reports / v.45, no.6, 2012 , pp. 348-353 More about this Journal
Abstract
L-2-Oxothiazolidine-4-carboxylic acid (OTC) is a cysteine prodrug that maintains glutathione in tissues. The present study was designed to investigate anti-fibrotic and anti-oxidative effects of OTC via modulation of nuclear factor erythroid 2-related factor 2 (Nrf2) in an in vivo thioacetamide (TAA)-induced hepatic fibrosis model. Treatment with OTC (80 or 160 mg/kg) improved serum liver function parameters and significantly ameliorated liver fibrosis. The OTC treatment groups exhibited significantly lower expression of ${\alpha}$-smooth muscle actin, transforming growth factor-${\beta}1$, and collagen ${\alpha}1$ mRNA than that in the TAA model group. Furthermore, the OTC treatment groups showed a significant decrease in hepatic malondialdehyde level compared to that in the TAA model group. Nrf2 and heme oxygenase-1 expression increased significantly in the OTC treatment groups compared with that in the TAA model group. Taken together, these results suggest that OTC restores the anti-oxidative system by upregulating Nrf2; thus, ameliorating liver injury and a fibrotic reaction.
Keywords
L-2-Oxothiazolidine-4-carboxylic acid; Liver fibrosis; Nrf2; Oxidative stress; Thioacetamide;
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