Browse > Article
http://dx.doi.org/10.5483/BMBRep.2012.45.6.025

Proteome analysis of developing mice diastema region  

Chae, Young-Mi (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
Jin, Young-Joo (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
Kim, Hyeng-Soo (School of Science and Biotechnology, Kyungpook National University)
Gwon, Gi-Jeong (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
Sohn, Wern-Joo (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
Kim, Sung-Hyun (Department of Center for Laboratory Animal Resources, Kyungpook National University)
Kim, Myoung-Ok (Department of Animal Science, Kyungpook National University)
Lee, Sang-Gyu (School of Science and Biotechnology, Kyungpook National University)
Suh, Jo-Young (Department of Periodontology, Kyungpook National University)
Kim, Jae-Young (Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University)
Publication Information
BMB Reports / v.45, no.6, 2012 , pp. 337-341 More about this Journal
Abstract
Different from humans, who have a continuous dentition of teeth, mice have only three molars and one incisor separated by a toothless region called the diastema in the hemi mandibular arch. Although tooth buds form in the embryonic diastema, they regress and do not develop into teeth. In this study, we evaluated the proteins that modulate the diastema formation through comparative analysis with molar-forming tissue by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) proteome analysis. From the comparative and semi-quantitative proteome analysis, we identified 147 up- and 173 down-regulated proteins in the diastema compared to the molar forming proteins. Based on this proteome analysis, we selected and evaluated two candidate proteins, EMERIN and RAB7A, as diastema tissue specific markers. This study provides the first list of proteins that were detected in the mouse embryonic diastema region, which will be useful to understand the mechanisms of tooth development.
Keywords
Diastema; EMERIN; RAB7A; Tooth development;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Sakurai, A., Maruyama, F., Funao, J., Nozawa, T., Aikawa, C., Okahashi, N., Shintani, S., Hamada, S., Ooshima, T. and Nakagawa, I. (2010) Specific behavior of intracellular Streptococcus pyogenes that has undergone autophagic degradation is associated with bacterial streptolysin O and host small G proteins Rab5 and Rab7. J. Biol. Chem. 285, 22666-22675.   DOI   ScienceOn
2 Irina Perdivara, I., Deterding, L. J., Przybylski, M. and Tomer, K. B. (2010) Mass spectrometric identification of oxidative modifications of tryptophan residues in proteins. J. Am. Soc. Mass. Spectrom. 21, 1114-1117.   DOI   ScienceOn
3 Gershon, D. (2005) Mass spectrometry: gaining mass appeal in proteomics. Nat. Methods. 2, 465-472.   DOI   ScienceOn
4 Bonne, G., Raffaele, M., Barletta, D., Varnous, S., Becane, H. M., Hammouda, E. H., Merlini, L., Muntoni, F., Greenberg, C. R., Gary, F., Urtizberea, J. A., Duboc, D., Fardeau, M., Toniolo, D. and Schwartz, K. (1999) Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy. Nature Genet 21, 285-288.   DOI   ScienceOn
5 Peralta, E. R., Martin, B. C. and Edinger, A. L. (2010) Differential effects of TBC1D15 and mammalian Vps39 on Rab7 activation state, lysosomal morphology, and growth factor dependence. J. Biol. Chem. 285, 16814-16821.   DOI   ScienceOn
6 Kinchen, J. M., Doukoumetzidis, K., Almendinger, J., Stergiou, L., Tosello-Trampont, A., Sifri, C. D., Hengartner, M. O. and Ravichandran, K. S. (2008) A pathway for phagosome maturation during engulfment of apoptotic cells. Nat. Cell Biol. 10, 556-566.   DOI   ScienceOn
7 Ohazama, A., Haycraft, C. J., Seppala, M., Blackburn, J., Ghafoor, S., Cobourne, M., Martinelli, D. C., Fan, C. M., Peterkova, R., Lesot, H., Yoder, B. K. and Sharpe, P. T. (2009) Primary cilia regulate shh activity in the control of molar tooth number. Development 136, 897-903.   DOI   ScienceOn
8 Seppala, M., Depew, M. J., Martinelli, D. C., Fan, C. M., Sharpe, P. T. and Cobourne, M. T. (2007) Gas1 is a modifier for holoprosencephaly and genetically interacts with sonic hedgehog. J. Clin. Invest. 117, 1575-1584.   DOI   ScienceOn
9 Andl, T., Reddy, S. T., Gaddapara, T. and Millar, S. E. (2002) Wnt signals are required for the initiation of hair follicle development. Dev. Cell. 2, 643-653.   DOI   ScienceOn
10 Ahn, Y., Sanderson, B. W., Klein, O. D. and Krumlauf, R. (2010) Inhibition of Wnt signaling by Wise (Sostdc1) and negative feedback from Shh controls tooth number and patterning. Development 137, 3221-3231.   DOI   ScienceOn
11 Li, L., Yuan, G., Liu, C., Zhang, L., Zhang, Y., Chen, Y. and Chen, Z. (2011) Exogenous fibroblast growth factor 8 rescues development of mouse diastemal vestigial tooth ex vivo. Dev. Dyn. 240, 1344-1353.   DOI   ScienceOn
12 Takano, M., Takeuchi, M., Ito, H., Furukawa, K., Sugimoto, K., Omata, S. and Horigome, K. (2002) The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region. Eur. J. Biochem. 269, 943-953.   DOI   ScienceOn
13 Peters, H. and Balling, R. (1999) Teeth-where and how to make them. Trends. Genet. 15, 59-65.   DOI   ScienceOn
14 Wang, X. and Fan, J. (2011) Molecular genetics of supernumerary tooth formation. Genesis 49, 261-277.   DOI   ScienceOn
15 Martin, A., Ochagavia, M. E., Rabasa, L. C., Miranda, J., Fernandez-de-Cossio, J. and Bringas, R. (2010) BisoGenet: a new tool for gene network building, visualization and analysis. BMC ioinformatics. 11, 91.   DOI   ScienceOn
16 Lee, K. K., Haraguchi, T., Lee, R. S., Koujin, T., Hiraoka, Y. and Wilson, K. L. (2001) Distinct functional domains in emerin bind lamin A and DNA-bridging protein BAF. J. Cell Sci.114, 4567-4573.
17 Ben, Y. R., Toutain, A., Arimura, T., Demay, L., Massart, C., Peccate, C., Muchir, A., Llense, S., Deburgrave, N., Leturcq, F., Litim, K. E., Rahmoun-Chiali, N., Richard, P., Babuty, D., Recan-Budiartha, D. and Bonne, G. (2007) Multitissular involvement in a family with LMNA and EMD mutations: Role of digenic mechanism. Neurology 68, 1883-1894.   DOI   ScienceOn
18 Peterkova, R., Peterka, M., Viriot, L. and Lesot, H. (2000) Dentition development and budding morphogenesis. J. Craniofac. Genet. Dev. Biol. 20, 158-172.
19 Turecková, J., Lesot, H., Vonesch, J. L., Peterka, M., Peterkova, R. and Ruch, J. V. (1996) Apotosis is involved in the disappearance of the diastemal dental primordia in mouse embryo. Int. J. Dev. Biol. 40, 483-489.
20 Yamamoto, H., Cho, S. W., Song , S. J., Hwang, H. J., Lee, M. J., Kim, J. Y. and Jung, H. S. (2005) Characteristic tissue interaction of the diastema region in mice. Arch. Oral. Biol. 50, 189-198.   DOI   ScienceOn
21 Yuan, G. H., Zhang, L., Zhang, Y. D., Fan, M. W., Bian, Z. and Chen, Z. (2008) Mesenchyme is responsible for tooth suppression in the mouse lower diastema. J. Dent. Res. 87, 386-390.   DOI   ScienceOn
22 Porntaveetus, T., Ohazama, A., Choi, H. Y., Herz, J. and Sharpe, P. T. (2011) Wnt signaling in the murine diastema. Eur. J. Orthod. 33, 1-7.   DOI   ScienceOn
23 Thesleff, I. and Sharpe, P. (1997) Signaling networks regulating dental development. Mech. Dev. 67, 111-123.   DOI   ScienceOn
24 Thesleff, I. and Aberg, T. (1999) Molecular regulation of tooth development. Bone 25, 123-125.   DOI   ScienceOn
25 Jernvall, J. and Thesleff, I. (2000) Reiterative signaling and patterning during mammalian tooth morphogenesis. Mech. Dev. 92, 19-29.   DOI   ScienceOn