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http://dx.doi.org/10.5483/BMBRep.2012.45.5.287

FGF-2 inhibits TNF-α mediated apoptosis through up-regulation of Bcl2-A1 and Bcl-xL in ATDC5 cells  

Kim, Hey-Ryun (Division of Biochemistry, College of Medicine, Chungbuk National University)
Heo, Youn-Moo (Division of Orthopaedic Surgery, College of Medicine, Chungbuk National University)
Jeong, Kyoung-Il (Division of Orthopaedic Surgery, College of Medicine, Chungbuk National University)
Kim, Yong-Min (Division of Orthopaedic Surgery, College of Medicine, Chungbuk National University)
Jang, Hae-Lan (Division of Medical Infomation and Management, College of Medicine, Chungbuk National University)
Lee, Kwang-Yeol (College of Pharmacy, Chonnam National University)
Yeo, Chang-Yeol (Department of Life Science and Division of Life and Pharmaceutical Sciences, Ewha Womans University)
Kim, Sung-Hoon (Genomic Informatics Center, Hankyong National University)
Lee, Hak-Kyo (Genomic Informatics Center, Hankyong National University)
Kim, Seung-Ryul (Division of Biochemistry, College of Medicine, Chungbuk National University)
Kim, Eung-Gook (Division of Biochemistry, College of Medicine, Chungbuk National University)
Choi, Joong-Kook (Division of Biochemistry, College of Medicine, Chungbuk National University)
Publication Information
BMB Reports / v.45, no.5, 2012 , pp. 287-292 More about this Journal
Abstract
FGF-2 is involved in cell survival, proliferation, apoptosis, and angiogenesis in a wide variety of cells. FRGRs, PI3K and MAP kinases are well known mediators of FGF signaling. Despite its known roles during many developmental processes, including osteogenesis, there are few known targets of FGF-2. In the present study, we identified Bcl2-A1 and Bcl-xL as two prominent targets involved in promoting cell survival. Pretreatment of ATDC5 cells with FGF-2 increased cell survival, while siRNAs specific for Bcl2-A1 and Bcl-xL compromised the anti-apoptotic effect of FGF-2, sensitized the cells to apoptosis triggered by TNF-${\alpha}$. Chemical inhibition of FGFR, NFkB, and PI3K activity by PD173074, pyrrolidine dithiocarbamate, and LY294002 respectively abrogated the FGF-2-mediated induction of Bcl2-A1 and Bcl-xL expression. Taken together, our data demonstrate that a subset of Bcl2 family proteins are the targets of FGF-2 signaling that promotes the survival of ATDC5 cells.
Keywords
Apoptosis; ATDC5 cells; Bcl-xL; Bcl2-A1; FGF-2; TNF-${\alpha}$;
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Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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