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http://dx.doi.org/10.5483/BMBRep.2012.45.3.159

The activation of CD99 inhibits cell-extracellular matrix adhesion by suppressing β1 integrin affinity  

Lee, Kyoung-Jin (Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University)
Lee, Sun-Hee (Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University)
Yadav, Birendra Kumar (Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University)
Ju, Hyun-Mi (Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University)
Kim, Min-Seo (Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University)
Park, Jeong-Hyun (Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University)
Jeoung, Doo-Il (Department of Biochemistry, Kangwon National University)
Lee, Han-Soo (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
Hahn, Jang-Hee (Department of Anatomy and Cell Biology, School of Medicine, Kangwon National University)
Publication Information
BMB Reports / v.45, no.3, 2012 , pp. 159-164 More about this Journal
Abstract
CD99 is known to be involved in the regulation of cell-cell adhesion. However, it remains unclear whether CD99 controls cell-extracellular matrix adhesion. In this study, the effects of CD99 activation on cell-extracellular matrix adhesion were investigated. It was found that engagement of CD99 with the stimulating antibody YG32 downregulated the adhesion of MCF-7 cells to fibronectin, laminin and collagen IV in a dose-dependent manner. The CD99 effect on cell-ECM adhesion was inhibited by overexpression of the dominant negative form of CD99 or CD99 siRNA transfection. Treatment of cells with $Mn^{2+}$ or by ${\beta}_1$ integrin-stimulating antibody restored the inhibitory effect of CD99 on cell-ECM adhesion. Cross-linking CD99 inactivated ${\beta}_1$ integrin through conformational change. CD99 activation caused dephosphorylation at Tyr-397 in FAK, which was restored by the ${\beta}_1$ stimulating antibody. Taken together, these results provide the first evidence that CD99 inhibits cell-extracellular matrix adhesion by suppressing ${\beta}_1$ integrin affinity.
Keywords
${\beta}_1$ integrin; CD99; Cell-ECM adhesion; Conformational change; FAK;
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