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http://dx.doi.org/10.5483/BMBRep.2012.45.2.108

Protopine reduces the inflammatory activity of lipopolysaccharide-stimulated murine macrophages  

Bae, Deok-Sung (Department of Biology Education, College of Education, Chungbuk National University)
Kim, Young-Hoon (Department of Biology Education, College of Education, Chungbuk National University)
Pan, Cheol-Ho (Functional Food Center, Korea Institute of Science and Technology)
Nho, Chu-Won (Functional Food Center, Korea Institute of Science and Technology)
Samdan, Javzan (Institutes of Chemistry and Chemical Technology)
Yansan, Jamyansan (Institutes of Chemistry and Chemical Technology)
Lee, Jae-Kwon (Department of Biology Education, College of Education, Chungbuk National University)
Publication Information
BMB Reports / v.45, no.2, 2012 , pp. 108-113 More about this Journal
Abstract
Protopine is an isoquinoline alkaloid contained in plants in northeast Asia. In this study, we investigated whether protopine derived from Hypecoum erectum L could suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Protopine was found to reduce nitric oxide (NO), cyclooxygenase-2 (COX-2), and prostaglandin $E_2$ ($PGE_2$) production by LPS-stimulated Raw 264.7 cells, without a cytotoxic effect. Pre-treatment of Raw 264.7 cells with protopine reduced the production of pro-inflammatory cytokines. These inhibitory effects were caused by blocking phosphorylation of mitogen-activated protein kinases (MAP kinases) and also blocking activation of a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-${\kappa}B$).
Keywords
Cyclooxygenase-2; Cytokine; Inflammation; Macrophage; Nitric oxide; Protopine;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
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