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http://dx.doi.org/10.5483/BMBRep.2012.45.1.20

Translationally controlled tumor protein (TCTP) downregulates Oct4 expression in mouse pluripotent cells  

Cheng, Xiang (Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University)
Li, Junhua (Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University)
Deng, Jie (Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University)
Li, Zhenzhen (Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University)
Meng, Shuyan (Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University)
Wang, Huayan (Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University)
Publication Information
BMB Reports / v.45, no.1, 2012 , pp. 20-25 More about this Journal
Abstract
The present study aimed to investigate the function of translationally controlled tumor protein (TCTP) in the regulation of Oct4 in mouse embryonic carcinoma P19 cells and mouse J1 embryonic stem (ES) cells. The mRNA level of endogenous TCTP in somatic cells was 2-4 folds higher than that in pluripotent P19 and J1 ES cells. Overexpression of TCTP in mouse pluripotent cells not only reduced the level of Oct4 transcription, but also decreased the pluripotency of stem cells. The N-terminal end of TCTP (amino acids 1-60) played an important role in suppressing the Oct4 promoter. Moreover, overexpression of TCTP in P19 cells suppressed the Oct4 promoter activity in a dose- and a time-dependent manner. In addition, knockdown of TCTP by small interfering RNA increased the expression of Oct4. Our study indicates that TCTP downregulates the Oct4 expression by binding the Sf1 site of Oct4 promoter in mouse pluripotent cells.
Keywords
Gene regulation; Oct4; Overexpression; siRNA interference; TCTP;
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1 Niwa, H. T. Y., Shimosato, D., Strumpf, D., Takahashi, K., Yagi, R. and Rossant, J. (2005 ) Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell 123, 917-929.   DOI   ScienceOn
2 Niwa, H. (2007) How is pluripotency determined and maintained? Development 134, 635-646.   DOI   ScienceOn
3 Johansson, H., Vizlin-Hodzic, D., Simonsson, T. and Simonsson, S. (2010) Translationally controlled tumor protein interacts with nucleophosmin during mitosis in ES cells. Cell Cycle 9, 2160-2169.   DOI   ScienceOn
4 Tuynder, M., Fiucci, G., Prieur, S., Lespagnol, A., Geant, A., Beaucourt, S., Duflaut, D., Besse, S., Susini, L., Cavarelli, J., Moras, D., Amson, R. and Telerman, A. (2004) Translationally controlled tumor protein is a target of tumor reversion. Proc. Natl. Acad. Sci. U.S.A. 101, 15364-15369.   DOI   ScienceOn
5 Chen, S. H., Wu, P. S., Chou, C. H., Yan, Y. T., Liu, H., Weng, S. Y. and Yang-Yen, H. F. (2007) A knockout mouse approach reveals that TCTP functions as an essential factor for cell proliferation and survival in a tissue- or cell type-specific manner. Mol. Biol. Cell 18, 2525-2532.   DOI   ScienceOn
6 Yarm, F. R. (2002) Plk phosphorylation regulates the microtubule- stabilizing protein TCTP. Mol. Cell Biol. 22, 6209-6221.   DOI   ScienceOn
7 Liou, J. Y., Ko, B. S. and Chang, T. C. (2010) An efficient transfection method for mouse embryonic stem cells. Methods Mol. Biol. 650, 145-153.   DOI   ScienceOn
8 Hummelke, G. C. and Cooney, A. J. (2001) Germ cell nuclear factor is a transcriptional repressor essential for embryonic development. Front Biosci. 6, D1186-1191.   DOI
9 Barnea, E. and Bergman, Y. (2000) Synergy of SF1 and RAR in activation of Oct-3/4 promoter. J. Biol. Chem. 275, 6608-6619.   DOI   ScienceOn
10 Gu, P. L., Goodwin, B., Chung, A. C. K., Xu, X. P., Wheeler, D. A., Price, R. R., Galardi, C., Peng, L., Latour, A. M., Koller, B. H., Gossen, J., Kliewer, S. A. and Cooney, A. J. (2005) Orphan nuclear receptor LRH-1 is required to maintain Oct4 expression at the epiblast stage of embryonic development. Mol. Cell Biol. 25, 3492-3505.   DOI   ScienceOn
11 Zhang, J., Tam, W. L., Tong, G. Q., Wu, Q., Chan, H. Y., Soh, B. S., Lou, Y., Yang, J., Ma, Y., Chai, L., Ng, H. H., Lufkin, T., Robson, P. and Lim, B. (2006) Sall4 modulates embryonic stem cell pluripotency and early embryonic development by the transcriptional regulation of Pou5f1. Nat. Cell Biol. 8, 1114-1123.   DOI   ScienceOn
12 Covello, K. L., Kehler, J., Yu, H., Gordan, J. D., Arsham, A. M., Hu, C. J., Labosky, P. A., Simon, M. C. and Keith, B. (2006) HIF-2alpha regulates Oct-4: effects of hypoxia on stem cell function, embryonic development, and tumor growth. Genes Dev. 20, 557-570.   DOI   ScienceOn
13 Koziol, M. J., Garrett, N. and Gurdon, J. B. (2007) Tpt1 activates transcription of Oct4 and nanog in transplanted somatic nuclei. Curr. Biol. 17, 801-807.   DOI   ScienceOn
14 Johansson, H. and Simonsson, S. (2010) Core transcription factors, Oct4, Sox2 and Nanog, individually form complexes with nucleophosmin (Npm1) to control embryonic stem (ES) cell fate determination. Aging (Albany NY) 2, 815-822.
15 Yeom, Y. I., Fuhrmann, G., Ovitt, C. E., Brehm, A., Ohbo, K., Gross, M., Hubner, K. and Scholer, H. R. (1996) Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells. Development 122, 881-894.
16 Okumura-Nakanishi, S., Saito, M., Niwa, H. and Ishikawa, F. (2005) Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells. J. Biol. Chem. 280, 5307-5317.   DOI   ScienceOn
17 Matin, M. M., Walsh, J. R., Gokhale, P. J., Draper, J. S., Bahrami, A. R., Morton, I., Moore, H. D. and Andrews, P. W. (2004) Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells. Stem Cells (Dayton, Ohio) 22, 659-668.   DOI   ScienceOn
18 Takahashi, K. and Yamanaka, S. (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.   DOI   ScienceOn
19 Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe- Nebenius, D., Chambers, I., Scholer, H. and Smith, A. (1998) Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95, 379-391.   DOI   ScienceOn
20 Niwa, H., Miyazaki, J. and Smith, A. G. (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet 24, 372-376.   DOI   ScienceOn
21 Shimozaki, K., Nakashima, K., Niwa, H. and Taga, T. (2003) Involvement of Oct3/4 in the enhancement of neuronal differentiation of ES cells in neurogenesis-inducing cultures. Development 130, 2505-2512.   DOI   ScienceOn
22 Bommer, U. A. and Thiele, B. J. (2004) The translationally controlled tumour protein (TCTP). Int. J. Biochem. Cell Biol. 36, 379-385.   DOI   ScienceOn
23 Susini, L., Besse, S., Duflaut, D., Lespagnol, A., Beekman, C., Fiucci, G., Atkinson, A. R., Busso, D., Poussin, P., Marine, J. C., Martinou, J. C., Cavarelli, J., Moras, D., Amson, R. and Telerman, A. (2008) TCTP protects from apoptotic cell death by antagonizing bax function. Cell Death Differ. 15, 1211-1220.   DOI   ScienceOn
24 Thaw, P., Baxter, N. J., Hounslow, A. M., Price, C., Waltho, J. P. and Craven, C. J. (2001) Structure of TCTP reveals unexpected relationship with guanine nucleotide-free chaperones. Nat. Struct. Biol. 8, 701-704.   DOI   ScienceOn