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http://dx.doi.org/10.5483/BMBRep.2012.45.12.058

p13 from group II baculoviruses is a killing-associated gene  

Lu, Nan (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
Du, Enqi (College of Veterinary Medicine, Northwest A&F University)
Liu, Yangkun (College of Veterinary Medicine, Northwest A&F University)
Qiao, Hong (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
Yao, Lunguang (China-UK, Nanyang Normal University-Rothamsted Research Joint Laboratory of Insect Biology, Nanyang Normal University)
Pan, Zishu (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
Lu, Songya (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
Qi, Yipeng (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
Publication Information
BMB Reports / v.45, no.12, 2012 , pp. 730-735 More about this Journal
Abstract
p13 gene was first described in Leucania separata multinuclear polyhedrosis virus (Ls-p13) several years ago, but the function of P13 protein has not been experimentally investigated to date. In this article, we indicated that the expression of p13 from Heliothis armigera single nucleocapsid nucleopolyhedrovirus (Ha-p13) was regulated by both early and late promoter. Luciferase assay demonstrated that the activity of Ha-p13 promoter with hr4 enhancer was more than 100 times in heterologous Sf9 cells than that in nature host Hz-AM1 cells. Both Ls-P13 and Ha-P13 are transmembrane proteins. Confocal microscopic analysis showed that both mainly located in the cytoplasm membrane at 48 h. Results of RNA interference indicated that Ha-p13 was a killing-associated gene for host insects H. armigera. The AcMNPV acquired the mentioned killing activity and markedly accelerate the killing rate when expressing Ls-p13. In conclusion, p13 is a killing associated gene in both homologous and heterologous nucleopolyhedrovirus.
Keywords
Autographa californica nucleopolyhedrovirus; Ha-p13; Heliothis armigera single nucleocapsid nucleopolyhedrovirus; Leucania separata multinuclear polyhedrosis virus; Ls-p13;
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